CPGWIZ^™ DAP-Kinase - Methylation specific PCR assay

FUNCTION: SwissProt: P51397 # Involved in mediating interferon-gamma-induced cell death.
SIZE: 102 amino acids; 11165 Da

Principles of the Technique

Use of either the CpGenome DNA Modification Kit (Cat. No. S7820) or the CpGenome Fast DNA Modification Kit (Cat. No. S7824) facilitates the initial bisulfite reactions, while the TM=["CPGWIZ"] DAP-kinase Amplification Kit contains the reagents required for the gene-specific PCR amplification reactions.

The components of the CPG WIZ DAP-kinase Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples with appropriate controls. U Primer Set7.5 μM each primer (25X) 35 μL (neutral cap) 90510 -15°C to -25°C M Primer Set7.5 μM each primer (25X) 35 μL (red cap) 90511 -15°C to -25°C W Primer Set7.5 μM each primer (25X) 35 μL (green cap) 90512 -15°C to -25°C U control DNA0.1 μg/μL 50 μL (white cap) 90393 -15°C to -25°C M control DNA0.1 μg/μL 50 μL (red cap) 90394 -15°C to -25°C W control DNA0.05 μg/μL 50 μL (green cap) 90395 -15°C to -25°C Universal 10X PCR Buffer 265 μL (blue cap) 90396 -15°C to -25°C

CPGWIZ is a trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.