CPGWIZ^™ ERα - Methylation specific PCR assay

Principles of the Technique
Use of either the CpGenome DNA Modification Kit (Cat. No. S7820) or the CpGenome Fast DNA Modification Kit (Cat. No. S7824) facilitates the initial bisulfite reactions, while the CPGWIZ^™ DAP-kinase Amplification Kit contains the reagents required for the gene-specific PCR amplification reactions.

FUNCTION: SwissProt: P03372 # Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
SIZE: 595 amino acids; 66216 Da
SUBUNIT: Binds DNA as a homodimer. Can form a heterodimer with ESR2. Interacts with NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NCOA7 in a ligand-inducible manner. Interacts with PHB2, PELP1 and UBE1C. Interacts with AKAP13. Interacts with CUEDC2. Interacts with JARID1A. Interacts with SMARD1. Interacts with SLC30A9 (By similarity). Interacts with HEXIM1 and MAP1S. Interacts with PBXIP1.
SUBCELLULAR LOCATION: Nucleus.
DOMAIN: SwissProt: P03372 Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.
PTM: Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
SIMILARITY: Belongs to the nuclear hormone receptor family. NR3 subfamily. & Contains 1 nuclear receptor DNA-binding domain.
MISCELLANEOUS: In the absence of ligand, steroid hormone receptors are thought to be weakly associated with nuclear components; hormone binding greatly increases receptor affinity. The hormone- receptor complex appears to recognize discrete DNA sequences upstream of transcriptional start sites.

The components of the CPG WIZ ERalpha Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples with appropriate controls. U Primer Set7.5 μM each primer (25X) / 35 μL (clear cap) / 90633 / -15°C to -25°C M Primer Set7.5 μM each primer (25X) / 35 μL (red cap) / 90634 / -15°C to -25°C W Primer Set7.5 μM each primer (25X) / 35 μL (green cap) / 90635 / -15°C to -25°C U control DNA0.1 μg/μL / 50 μL (clear cap) / 90393 / -15°C to -25°C M control DNA0.1 μg/μL / 50 μL (red cap) / 90394 / -15°C to -25°C W control DNA0.05 μg/μL / 50 μL (green cap) / 90395 / -15°C to -25°C Universal 10X PCR Buffer / 265 μL (blue cap) / 90396 / -15°C to -25°C

CPGWIZ is a trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.