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Merck
CN

SCC007

ReNcell CX人神经祖细胞系

ReNcell CX is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons & glial cells.

别名:

Human NSC Line, Human Neural Stem Cell Line, Human Neural Stem Cells

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关于此项目

UNSPSC Code:
41106509
NACRES:
NA.75
eCl@ss:
32011203
Biological source:
human
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产品名称

ReNcell CX人神经祖细胞系, ReNcell CX is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons & glial cells.

biological source

human

manufacturer/tradename

Chemicon®
ReNcell

technique(s)

cell culture | stem cell: suitable

input

sample type neural stem cell(s)

shipped in

liquid nitrogen

Quality Level

Analysis Note

已经验证了每批ReNcell CX细胞的巢蛋白和Sox 2的高水平表达及其自我更新和多谱系分化能力(请参见数据表图)。 通过染色体扩散评估,细胞还显示出正常的核型,并且支原体测试为阴性。已对孕妇血液进行了HIV、HTLV、乙型和丙型肝炎筛查。

Application

研究类别
干细胞研究

Biochem/physiol Actions

神经干细胞

Disclaimer

仅供研究使用。在法国,在用于科研目的时(包括进口和出口活动(《公共健康法》第L 1211-1条,第2节))该产品为管制品。购买者(即最终用户)需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。

General description

ReNcell CX是一种永生化的人神经祖细胞系,具有易于分化为神经元和神经胶质细胞的能力。 ReNcell CX源自人胎脑的皮质区域。 通过用c-myc癌基因进行逆转录病毒转导而永生化,该细胞系在层粘连蛋白上以单层快速生长,倍增时间为20-30小时。 核型分析表明,即使经过长时间传代(>45代),ReNcell CX仍可在培养物中保留正常的二倍体核型。 ReNcell CX由ReNeuron Group plc开发,ReNeuron Group plc是一家专门将人体体干细胞用于治疗的生物技术公司。 ReNcell CX可用于多种研究应用,例如神经毒性、神经发生、电生理学、神经递质和受体功能的研究。 ReNcell CX细胞是按照法律和伦理的方式获得的,符合现行的当地知情同意程序。

ReNcell现在用于阿尔茨海默′病的3D细胞模型!
Tanzi, R and Kim, DY et. al. A three-dimensional human neural cell culture model of Alzheimer′s disease.Nature.2014 Nov 13;515(7526):274-8.
发表在Nature上
产品来源:人类皮质脑组织

Other Notes

> 1x106 活ReNcell CX细胞
根据产品文件中详述的“学术使用协议”的条款,本产品预期仅用于销售和销售给学术机构,以供内部学术研究使用。有关任何其他用途的信息,请联系licensing@emdmillipore.com。

Preparation Note

当在推荐的储存条件(液氮)下储存时,ReNcell CX细胞在有效期内保持稳定。 请勿暴露于高温下。 到期后,请丢弃所有剩余的试剂。 我们建议在十代内使用细胞。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

存储类别

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

does not flash

flash_point_c

does not flash

法规信息

高风险级别生物产品-Merck
此项目有

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Mia C Borlongan et al.
Neuromolecular medicine, 23(4), 540-548 (2021-04-09)
The present in vitro study showed that IL-2/IL-2R antibody complex facilitates Treg-induced neuroprotection in the oxygen glucose deprivation/reoxygenation (OGD/R) model of stroke. First, we examined the role of IL-2/IL-2R-treated Tregs in OGD/R-exposed rat primary cortical cells (PCCs), which represent the
Greg Maguire et al.
Physiological reports, 7(9), e14072-e14072 (2019-05-03)
Evidence suggests that adult stem cell types and progenitor cells act collectively in a given tissue to maintain and heal organs, such as muscle, through a release of a multitude of molecules packaged into exosomes from the different cell types.
Qi Gu et al.
Advanced healthcare materials, 5(12), 1429-1438 (2016-03-31)
Direct-write printing of stem cells within biomaterials presents an opportunity to engineer tissue for in vitro modeling and regenerative medicine. Here, a first example of constructing neural tissue by printing human neural stem cells that are differentiated in situ to
Liang Luo et al.
Cells, 10(3) (2021-03-07)
Human adipose-derived stromal cells (ADSCs) are receiving unprecedented attention as a potential cellular source for regenerative medicine-based therapies against various diseases and conditions. However, there still have significant issues concerning the translational development of ADSC-based therapies, such as its heterogeneity
Nathalia Barth de Oliveira et al.
Membranes, 11(9) (2021-09-27)
Adipose tissue-derived mesenchymal stem cells (ADMSCs) are promising candidates for regenerative medicine, as they have good cell yield and can differentiate into several cell lines. When induced to the neuronal differentiation, they form neurospheres composed of neural precursors (NPs) that

商品

Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.

Frequently asked questions about neural stem cells including NSC derivation, expansion and differentiation.

ReNcell neural progenitors are immortalized human neural stem cell lines that can differentiate into neurons, astrocytes sand oligodendrocytes.

Read types of stem cells including multipotent stem cells, Pluripotent stem cells and iPSCs and their applications in basic stem cell research, stem cell therapy and disease modelling

实验方案

A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

相关内容

ReNcell® VM and ReNcell® CX are two well-established neural stem cell lines derived from developing human brains. ReNcell® VM and CX cells are generated from the ventral mesencephalon and cortical regions of the brain, respectively, and transduced with the myc transcription factor. Both cell lines offer phenotype and genotype stability, in addition to the multipotential neuronal differentiation capacity, over long-term culture. This article describes the characteristics and differentiation of ReNcell® lines. Results of calcium and membrane potential changes in response to various ligands are also shown. The convenience to maintain in culture and flexibility to differentiate to individual scientists’ needs make ReNcell® lines the ideal platform for research and discovery.

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