ReNcell® Human Neural Progenitor Cell Line (ReNSC5)

Understanding human neurodevelopment and the underlying biology remains a complex issue. Stem cell biology improvements have introduced new tools and more useful model systems. Human neural stem cells (hNSCs) and neural progenitor cells (NPCs) have provided a robust system in which to investigate the complex neurobiology questions in modern science. NPCs have the capability to differentiate into different neural cell types such as astrocytes and oligodendrocytes. They are useful in screening new, potentially therapeutic drugs in a highly cost-efficient and high-throughput manner due to the reliable and robust source of cells. NPCs can also be easily manipulated, making them an ideal platform to use.1 The ReNcell® Human Neural Progenitor group of cell lines are conditionally immortalized human neural progenitor cells which appropriately retain characteristics of their original tissue sites. Furthermore, the cells are genetically modified with a chemical switch (conditional immortalization) to give a greater control of proliferation and differentiation. While these cells are grown in their progenitor state, they can be terminally differentiated into neurons with distinct characteristics reflective of their original brain regions. This makes the ReNcell® hNPCs a strong source of distinct neural cells in which to further investigate neurobiology. Each line was conditionally immortalized using retroviral integration of a single copy of the c-mycERTAM construct.1 This allows the cells to retain proliferative properties when grown with the necessary growth factors and 4-hydroxytamoxifen (4-OHT). With changes in media formulation, the cells start to differentiate into mature neurons characteristic of their original tissue region. The ReNSC5 cell line is originally sourced from human ventral mesencephalon. The ReNcell® hNPC lines have a high number of potential applications in which they can be utilized such as disease modelling, drug discovery, neural cytotoxicity testing, and target validation.

These cells should be stored in liquid nitrogen.

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

1.Anderson GW, Deans M, Taylor RDT, Raval P, Chen D, Lowder H, Srishti Murkerji, Andreae LC, Williams BP, Srivastava DP. 2015. Characterisation of neurons derived from a cortical human neural stem cell line CTX0E16. Stem Cell Research & Therapy. 6(1). doi:https://doi.org/10.1186/s13287-015-0136-8. [accessed 2024 Sep 26]. https://stemcellres.biomedcentral.com/articles/10.1186/s13287-015-0136-8.2.Jeffries AR, Perfect LW, Ledderose J, Schalkwyk LC, Bray NJ, Mill J, Price J. 2012. Stochastic Choice of Allelic Expression in Human Neural Stem Cells. STEM CELLS. 30(9):1938–1947. doi:https://doi.org/10.1002/stem.1155.3.Anacker C, Zunszain PA, Cattaneo A, Carvalho LA, Garabedian MJ, Thuret S, Price J, Pariante CM. 2011. Antidepressants increase human hippocampal neurogenesis by activating the glucocorticoid receptor. Molecular Psychiatry. 16(7):738–750. doi:https://doi.org/10.1038/mp.2011.26.4.Miljan EA, Hines SJ, Pande P, Corteling RL, Hicks C, Zbarsky V, Umachandran M, Sowinski P, Richardson S, Tang E, et al. 2009. Implantation of c-mycERTAMImmortalized Human Mesencephalic-Derived Clonal Cell Lines Ameliorates Behavior Dysfunction in a Rat Model of Parkinson′s Disease. Stem Cells and Development. 18(2):307–320. doi:https://doi.org/10.1089/scd.2008.0078.5.Amemori T, Romanyuk N, Jendelova P, Herynek V, Turnovcova K, Prochazka P, Kapcalova M, Cocks G, Price J, Sykova E. 2013. Human conditionally immortalized neural stem cells improve locomotor function after spinal cord injury in the rat. Stem Cell Research & Therapy. 4(3):68. doi:https://doi.org/10.1186/scrt219.

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacture a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact licensing@milliporesigma.com

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.