CH12.LX Mouse Lymphoma Cell Line

Naïve B-cells differentiate and expand upon antigenic stimulation, undergoing class-switch recombination of immunoglobulin genes and forming antibody-producing plasma cells and memory cells that generate upon recall stimulation (1). B-cell models are useful for elucidating pathways of differentiation and proliferation mechanisms of lymphoma.
The CH12.LX mouse lymphoma cell line is an established and well-characterized B-cell model. CH12.LX cells express Ly-12 and have the characteristic IgM+CD5+CD23-cell surface marker expression of B1a B lymphocytes, as well as expressing CD19, CD11b, MHC II and alpha-4-integrin (3). CH12.LX cells are responsive to a variety of antigens including lipopolysaccharide, expressing the activation protein CD14 (4). CH12.LX cells proliferate rapidly and are ideal for studies of B-cell activation and expansion.

<bold>Source:</bold>
The CH12.LX cell line was generated from B10.H-2aH4bp/Wts mice immunized with sheep erythrocytes (2).

Research Category:
Cancer

Mouse, Cancer Cells

&ge;1X10⁶ cells/vial

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

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