description
Cell line type: Cancer Cells
technique(s)
cell culture | mammalian: suitable
General description
Ovarian carcinoma is a prevalent worldwide disease, with over 200,000 new cases diagnosed per year (1). Metastatic gastrointestinal cancer frequently presents as advanced ovarian carcinoma (2). The availability of cellular models that recapitulate the spectrum of forms of ovarian cancer are essential to understanding drug resistance, cellular physiology, and advancing new options for treatment.
The OVCAR-5 cell line was established from ascites fluid from a non-treated patient with an advanced-stage ovarian tumor (3). The OVCAR-5 cell line has recently been classified as originating from the gastrointestinal tract, not of ovarian origin (4). OVCAR-5 cells harbor a homozygous Gly12Val mutation in the KRAS oncogene and are characterized by migration/invasion ability as well as exhibiting tumorigenicity in nude mice (5). OVCAR-5 cells overexpress claudin-4, a marker that plays a role in cancer malignancy (6). OVCAR-5 cells exhibit aggressive growth and invasion and represent a valuable model for metastatic gastrointestinal carcinoma.
Source:
OVCAR-5 was established from ascites fluid from a 67-year-old untreated cancer patient (3).
References:
1. Nat Rev Disease Primers 2016; 2: 16061.
2. J Obstet Gynaecol Can 2003; 25(10): 819-824.
3. Cancer Res 1997; 57: 850-856.
4. Nuc Acids Res 2016;44(17): e137.
5. Gynecol Oncol 2015;138(2): 372-377.
6. Int J Mol Sci 2011; 12: 1334-1358.
The OVCAR-5 cell line was established from ascites fluid from a non-treated patient with an advanced-stage ovarian tumor (3). The OVCAR-5 cell line has recently been classified as originating from the gastrointestinal tract, not of ovarian origin (4). OVCAR-5 cells harbor a homozygous Gly12Val mutation in the KRAS oncogene and are characterized by migration/invasion ability as well as exhibiting tumorigenicity in nude mice (5). OVCAR-5 cells overexpress claudin-4, a marker that plays a role in cancer malignancy (6). OVCAR-5 cells exhibit aggressive growth and invasion and represent a valuable model for metastatic gastrointestinal carcinoma.
Source:
OVCAR-5 was established from ascites fluid from a 67-year-old untreated cancer patient (3).
References:
1. Nat Rev Disease Primers 2016; 2: 16061.
2. J Obstet Gynaecol Can 2003; 25(10): 819-824.
3. Cancer Res 1997; 57: 850-856.
4. Nuc Acids Res 2016;44(17): e137.
5. Gynecol Oncol 2015;138(2): 372-377.
6. Int J Mol Sci 2011; 12: 1334-1358.
Application
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact licensing@milliporesigma.com.
Preparation Note
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Analysis Note
- Each vial contains >=1X106 viable cells.
- Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
- Cells are verified to be of human origin and negative for inter-species contamination from rat, mouse, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
- Cells are negative for mycoplasma contamination.
- Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.
存储类别
10 - Combustible liquids
WGK
WGK 1
闪点 (F)
Not applicable
闪点 (°C)
Not applicable