MCF-7 BUS Human Invasive Ductal Carcinoma Cell Line

Each vial contains ≥1X10⁶ viable cells.Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.Cells are verified to be of human origin and negative for inter-species contamination from mouse, rat, Chinese hamster, Golden Syrian hamster, and Non-human Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services Cells are negative for mycoplasma contamination.

Hormonal response is an important characteristic of mammary cell growth and differentiation (1). Malignant mammary epithelial cells expressing estrogen receptor (ER) may exhibit enhanced proliferation in response to estrogen hormones, underscoring the necessity of research on cellular models recapitulating this trait and helping to inform the most effective avenues of therapeutic development for ER-positive breast cancers. MCF7 was one of the first established breast cancer cell lines, retaining characteristics of differentiated mammary epithelium, such as processing of estradiol via cytoplasmic estrogen receptors (2). MCF7-BUS is a subline of MCF7 that demonstrates the highest proliferative response to estradiol-17β (E2) among MCF7 sublines (3,4). MCF7-BUS is a slow-growing line with tendency to form clusters in culture. MCF7-BUS cells have been validated for dose-response to estradiol. MCF7-BUS represents a clinically applicable and well-characterized cellular model for estrogen-responsive metastatic breast cancer.MCF7-BUS is a subclone of the MCF7 cell line isolated from pleural effusion from a 69-year -old female patient with metastatic breast cancer (2).1. Endocrinology 2002, 143(9): 3427-3434.2. J Natl Cancer Inst 1973, 51(5): 1409-1416.3. Best Pract Res Clin Endocrinol Metab 2006, 20(1): 15-33.4. Environ Heath Perspect 1995, 103(9): 844-850.

The MCF-7 BUS Human Invasive Ductal Carcinoma Cell Line was subcloned from the MCF-7 cell line. The parental MCF-7 cell line was isolated from a metastatic human breast cancer patient and is an adherent, epithelial luminal cell line positive for estrogen and progesterone receptors. MCF-7 BUS shows the highest proliferative response to estradiol-17β (E2) among MCF7 stocks.References:Soto, A. M., et al. (2006). Strengths and weaknesses of in vitro assays for estrogenic and androgenic activity. Best Practice & Research Clinical Endocrinology & Metabolism, 20(1), 15-33.Soto, A. M., et al. (2004). Androgenic and estrogenic activity in water bodies receiving cattle feedlot effluent in Eastern Nebraska, USA. Environmental health perspectives, 112(3), 346-352.

MCF-7 BUS is a well-characterized cellular model for estrogen-responsive metastatic breast cancer.

Cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

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RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.