U2OS EGFP-H2B Human Osteosarcoma Cell Line

Chromosomal abnormalities underlie a substantial proportion of cancers, including most solid tumors (1). Chromosome instability manifests in a range of defects, from aneuploidy (abnormal number of entire chromosomes), to chromosomal breakage and rearrange

Chromosomal abnormalities underlie a substantial proportion of cancers, including the majority of solid tumors.1 Chromosome instability manifests in a range of defects, from aneuploidy (abnormal number of entire chromosomes), to chromosomal breakage and rearrangements.1 Understanding the mechanisms of chromosomal instability is key to unlocking the multifaceted roles that they play in cancer etiology and progression. The U2OS osteosarcoma is one of the first and most widely used cancer cell lines, especially for the study of mitosis, apoptosis, and the cellular mechanisms that give rise to cancer. U2OS cells undergo chromosome breakage and chromothripsis at high frequency.2 The expression of EGFP fused to a histone marker in the U2OS EGFP-H2B cell line allows for visualization of chromosome dynamics by light microscopy, a powerful tool in unraveling the complex interactions and molecular players involved in chromosomal instability.3 Expression of EGFP-H2B in this cell line is maintained via selection with blasticidin. The U2OS EGFP-H2B cell line facilitates expanded insights to chromosomal biology, allowing immediate relevancy to the many studies using the well-characterized U2OS background.Source The U2OS EGFP-H2B Human Osteosarcoma cell line was derived from U2OS cells stably transfected with a construct expressing EGFP-histone-H2B.3 The parental U2OS cell line was derived from an osteosarcoma from a 15-year-old female.2References1. Thompson SL, Compton DA. Chormosome Res. 2011; 19(3):433-444.2. Pontén J, Saksela E. Int J Cancer. 1967; 2(5):434-447.3. Wang H et al. Oncotarget 2017; 8(30):48671-48687.

Each vial contains ≥ 1X10⁶ viable cells.Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.Cells are verified to be of human origin and negative for inter-species contamination from mouse, rat, Chinese hamster, Golden Syrian hamster, and Non-human Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services Cells are negative for mycoplasma contamination.

U2OS EGFP-H2B Human Osteosarcoma Cell Line allows for visualization of chromosome dynamics by light microscopy

The cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact licensing@milliporesigma.com.

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.