biological source
human (dermal fibroblast)
description
Cryopreserved human iPSC derived anterior foregut endoderm (AFE) progenitor cells that express Sox2, Pax9 and EpCAM.
packaging
vial of >1500 viable organoids
gender
male
technique(s)
cell culture: suitable
application(s)
3D cell culture
control
(dermal fibroblast)
relevant disease(s)
(Normal)
storage temp.
−196°C
General description
The anterior foregut endoderm (AFE) gives rise to therapeutically relevant cell types in tissues such as the lung, esophagus, salivary glands, thymus, parathyroid and thyroid. Our cryopreserved anterior foregut endoderm (AFE) progenitor cells were derived from integration-free human iPS cells (Cat. No. SCC271) reprogrammed using the Simplicon RNA reprogramming technology (Cat. No. SCR550) in chemically defined/serum-free culture conditions. The AFE cells are double positive for Sox2 (A,C; Sigma AB5603A4; 1:50), Pax9 (B, C; Abcam ab28538; 1:200) and approximately 20-30% are double positive for EpCAM (D, F; Sigma MAB4444; 1:500) and Pax9 (E, F).
The human iPSC derived AFE progenitors are a component of the 3dGRO® Human Lung Organoid Culture System which can be used to differentiate human iPS cells into mature lung organoids that structurally resemble the <italic>in vivo</italic> branching airway and early alveolar structures. The 3dGRO® Human Lung Organoid Culture System includes (all sold separately):
Research Category:
The human iPSC derived AFE progenitors are a component of the 3dGRO® Human Lung Organoid Culture System which can be used to differentiate human iPS cells into mature lung organoids that structurally resemble the <italic>in vivo</italic> branching airway and early alveolar structures. The 3dGRO® Human Lung Organoid Culture System includes (all sold separately):
- Definitive Endoderm Induction Medium (SCM302)
- Anterior Foregut Endoderm Induction Medium I (SCM305)
- Anterior Foregut Endoderm Induction Medium II (SCM306)
- 3dGRO® Lung Organoid Branching Medium (SCM307)
- 3dGRO® Lung Organoid Maturation Medium (SCM308)
- Human iPSC Derived AFE Progenitors (SCC301)
Research Category:
- Cell Culture
- Stem Cell Research
Packaging
3X106 cell/vial
Preparation Note
Human iPSC Derived AFE Progenitors should be stored in liquid nitrogen.
Legal Information
3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany
3dGRO® is a trademark of Merck KGaA, Darmstadt, Germany.
3dGRO® organoids were derived utilizing HUB Organoid Technology.
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/pdouse. This product may not be re-engineered or copied; used to make derivatives or modifications; used to develop, test, or manufacture a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact organoids@milliporesigma.com.
3dGRO® organoids were derived utilizing HUB Organoid Technology.
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/pdouse. This product may not be re-engineered or copied; used to make derivatives or modifications; used to develop, test, or manufacture a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact organoids@milliporesigma.com.
存储类别
10 - Combustible liquids
怎么回事？
WGK 3
闪点 (F)
Not applicable
闪点 (°C)
Not applicable