MO-91 Acute Myeloid Leukemia w Minimal Differentiation Human Cell Line

AML (acute myeloid leukemia) is caused by mutations of the genes involved in hematopoiesis. These mutations result in clonal expansion of myeloid progenitors known as blasts in the bone marrow and peripheral blood. This leads to ineffective erythropoiesis and bone marrow dysfunction. ?
MO-91 is the first cell line identified to express the TEL-TRKC fusion gene from its endogenous promoter. It is therefore a valuable cell model for the screening of the TRKC inhibitor, and for the study of hematological and non-hematological diseases associated with theTEL-TRKC fusion gene. ?


SOURCE

The MO-91 cell line was established from a patient with acute myeloid leukemia with minimal differentiation (AML-M0)



1. Vakiti A, Mewawalla P. Acute Myeloid Leukemia. [Updated 2023 Aug 8]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2023 Jan-. Available at https://www.ncbi.nlm.nih.gov/books/NBK507875/

2. Gu, TL., Popova, L., Reeves, C. et al. Phosphoproteomic analysis identifies the M0-91 cell line as a cellular model for the study of TEL-TRKC fusion-associated leukemia. Leukemia 21, 563–566 (2007). https://doi.org/10.1038/sj.leu.2404555

3. Venditti, A et al. “Minimally differentiated acute myeloid leukemia (AML-M0): comparison of 25 cases with other French-American-British subtypes.” Blood vol. 89,2 (1997): 621-9.

Acute myeloid leukemia with minimal differentiation (AML-M0) is a rare subtype of leukemia in which myeloid blasts fail to show morphologic differentiation. SCC618, the MO-91 cell line, is the first cell line identified to express the TEL-TRKC fusion gene from its endogenous promoter. It is therefore a valuable cell model for the screening of the TRKC inhibitor and for the study of hematological and non-hematological diseases associated with the TEL-TRKC oncoprotein.Note: MO-91 is a suspension cell line, but has both suspension and adherent characteristics when growing in tissue culture-treated plasticware lying flat. The monolayer can be dislodged into single cells by pipetting with a 10 mL pipet. No dissociation with Accutase or other reagent is needed. Cells may be cultured in TC-treated flask by placing flask in upright position. Cells at the bottom 1/3 of the upright flask may require more effort to dislodge.

Cells are tested negative for infectious diseases by a Human Essential CLEAR Panel by Charles River Animal Diagnostic Services.
Cells are verified to be of human origin and negative for inter-species contamination from mouse, rat, Chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
Cells are negative for mycoplasma contamination.

Phosphoproteomic analysis has identified the MO-91 human acute myeloid leukemia cell line as a suitable cellular model for the study of TEL-TRKC fusion-associated leukemia, as well as a model of M0 (minimally differentiated) acute myeloid leukemia, or AML.

SCC618 cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting functionality.

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