Jurkat JE6.1 NF-kB::eGFP hTLR2/6 (human Toll-like Receptor 2/6) Cell Line

Contamination with bacterial components is a potential issue with the biologics for research and therapeutic uses, as they can activate the so-called pattern-recognition receptors in mammalian cells and trigger immune responses, clouding the experimental results or even causing health hazards. Such responses are often mediated by the Toll-Like Receptors (TLRs), which recognize bacterial molecular patterns to activate, among other things, cytokine expression through the NF-kB pathway.

The current assessment method for bacterial contamination measures lipopolysaccharide (LPS), the active component of endotoxins. Endotoxin measurement relies on its reaction with the limulus amoebocyte lysate (LAL), derived from the horseshoe crab blood.3 While the method is well established, however, the use of primary lysate obtained from wild animals presents a major sustainability issue.4 Recombinant alternatives to the primary LAL method have recently been gaining acceptance, but all LAL-based methods measure only the endotoxin contamination, and other classes of bacterial components go undetected.

To address these unmet needs, the Jurkat JE6.1 cell line has been engineered to form a group of cell lines, each expressing a specific set of TLRs and harboring an eGFP expression cassette fused to the NF-kB Response Element. The result is that each of these strains expresses eGFP in response to a specific class of bacterial molecules.

SCC632, Jurkat JE6.1 NF-kB::eGFP hTLR2/6 cell line, expresses recombinant TLR2 and TLR6 on its surface and is designed to express eGFP in response diacyl lipopeptides such as FSL-1. It is not responsive to other bacterial components, such as LPS (ligand for SCC630, TLR4 expressing), and Flagellin (ligand for SCC631, TLR5 expressing). It has also been tested negative for PAM3CSK4 (ligand for TLR2/TLR1).1

Source
SCC632 was retrovirally transduced with TLR2. TLR2 and the endogenous TLR6 form functional complexes. Homodimers of TLR2 or TLR6 do not react with the commonly tested bacterial components, and the strain is specific to the TLR2/TLR6 ligands.

Jurkat JE6.1 NF-ĸB::eGFP hTLR2/6 cell line, expresses recombinant TLR2 and TLR6 on its surface and is designed to express eGFP in response diacyl lipopeptides such as FSL-1.

Jurkat JE6.1 T NF-kB::eGFP hTLR2/6 cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages without significantly affecting cell marker expression and function.

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@milliporesigma.com.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.