产品名称
EmbryoMax® MEF Conditioned Media, EmbryoMax MEF Conditioned Media (MEF-CM) can be used for the feeder-free culture of human ES/iPS cells.
manufacturer/tradename
Specialty Media
EmbryoMax®
technique(s)
cell culture | stem cell: suitable
shipped in
ambient
Quality Level
Analysis Note
Mycoplasma Testing: PASSED
Bacterial Testing: PASSED
Fungi Testing: PASSED
Bacterial Testing: PASSED
Fungi Testing: PASSED
Biochem/physiol Actions
Primary Mouse Embryo Fibroblasts
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Traditionally, human embryonic stem cell (hESC) or induced pluripotent stem cell (iPSC) culture require mouse embryonic fibroblasts (MEF) support. This requirement makes the protocol complicated and variable. MEF conditioned medium (MEFCM) contains all the growth factors needed in hESC/iPSC culture, eliminating the routine preparation of feeder cells. It is a ready-to-use product for the feeder-free culture of human ES/IPS cells.
Each batch of EmbryoMax MEF Conditioned Media has been tested for hES/iPS cells pluripotency. The media requires an additional 10 ng/ml of bFGF to be added once thawed before use.
Each batch of EmbryoMax MEF Conditioned Media has been tested for hES/iPS cells pluripotency. The media requires an additional 10 ng/ml of bFGF to be added once thawed before use.
Preparation Note
Store at -80C until expiration date on label.
Legal Information
EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
实验方案
Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.
产生人诱导多能干细胞(iPSC)的干细胞重编程实验方案,包括基于病毒和非病毒RNA的方法。
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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