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SCR004

碱性磷酸酶检测试剂盒

This Alkaline Phosphatase Detection Kit is a specific & sensitive tool for the phenotypic assessment of Embryonic Stem (ES) cell differentiation by the determination of AP activity.

别名:

ALP Stain, Alkaline Phosphtase Stem Cell Staining Kit, Stem Cell AP Staining Kit

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UNSPSC Code:
12352207
NACRES:
NA.84
eCl@ss:
32161000

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产品名称

碱性磷酸酶检测试剂盒, This Alkaline Phosphatase Detection Kit is a specific & sensitive tool for the phenotypic assessment of Embryonic Stem (ES) cell differentiation by the determination of AP activity.

species reactivity (predicted by homology)

mammals

manufacturer/tradename

Chemicon®

technique(s)

cell culture | stem cell: suitable

input

sample type cardiac stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)
sample type mesenchymal stem cell(s)
sample type: human embryonic stem cell(s)

shipped in

wet ice

Quality Level

100

相关类别

Application

研究类别
干细胞研究
胚胎干（ES）细胞是来自胚胎植入前哺乳动物胚胎内部细胞团（ICM）的全能细胞，能够在体外无限、未分化增殖[1,2,3]。 未分化的鼠类ES细胞可以在含有细胞因子、白血病抑制因子（LIF）或Millipore专有的ES细胞培养试剂ESGRO的培养基中实现长时间的体外维持′ [4,5]。 ES细胞的未分化状态表现为碱性磷酸酶（AP）的高水平表达[6]、包括SSEA和TRA抗原的表面标志物以及转录因子Oct-4的表达。

Millipore′碱性磷酸酶检测试剂盒（货号SCR004）是一种特异性和灵敏的工具，可通过确定AP活性来对ES细胞分化进行表型评估。

可从Millipore单独购买单克隆抗体TRA-2-49（产品货号MAB4349）和TRA-2-54（产品货号MAB4354），其可用于检测ES细胞表达的肝/骨/肾AP同工酶。 当用于流式细胞术时，这些试剂可以定量评估ES细胞对AP表达的影响[7]。

仅限研究用途，不可用于诊断

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

Millipore′碱性磷酸酶检测试剂盒（货号SCR004）是一种特异性和灵敏的工具，可通过确定AP活性来对ES细胞分化进行表型评估。

Other Notes

快速红色紫罗兰色溶液（0.8 g/L储存液）（产品号90239）。两个15 mL瓶。

在AMPD缓冲液（pH 9.5;2 mol/L）中的Napthol AS-BI磷酸盐溶液（4 mg/mL）（产品号90234）。一个15 mL瓶。

Preparation Note

碱性磷酸酶检测试剂盒由用于确定AP活性的两个组分组成。

当存储在2-8º C，试剂盒的组分可在有效期内一直保持稳定。 请勿冷冻或暴露在高温下。到期后，请丢弃所有剩余的试剂。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

Hazard Classifications

Carc. 2 - Eye Irrit. 2 - Skin Irrit. 2

存储类别

10 - Combustible liquids

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Ahmed K Elsayed et al.
Stem cell research, 42, 101705-101705 (2020-01-29)
FOXA2 is a transcription factor, playing an important role during development. We established an induced pluripotent stem cell (iPSC) line, QBRIi009-A, using non-integrating Sendai virus from a 4-year-old boy, displaying a complex clinical phenotype. Molecular karyotyping and cytogenetics confirmed a
Efficient iPS cell production with the MyoD transactivation domain in serum-free culture.
Hiroyuki Hirai,Nobuko Katoku-Kikyo,Peter Karian,Meri Firpo,Nobuaki Kikyo
Testing null
Feeder-independent culture of mouse embryonic stem cells using vitamin A/retinol.
Jaspal S Khillan,Liguo Chen
Methods in Molecular Biology null
The ROCK inhibitor Y-27632 enhances the survival rate of human embryonic stem cells following cryopreservation.
Xiangyun Li, Guoliang Meng, Roman Krawetz, Shiying Liu, Derrick E Rancourt
Stem Cells and Development null
A novel method for generating xeno-free human feeder cells for human embryonic stem cell culture.
Guoliang Meng, Shiying Liu, Roman Krawetz, Michael Chan, Judy Chernos, Derrick E Rancourt
Stem Cells and Development null
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The cell culture protocols described in this instructional manual include the in vitro culture of murine ES cells using EmbryoMax products along with ESGRO® mLIF medium supplement, as well as feeder-free and serum-free ES cell culture using the ESGRO Complete™ line of products. Also included in this instructional manual are: methods for serum-free neuronal differentiation of mouse ES cells, iPS cell generation using STEMCCA™ lentivirus kits, cre-recombinase mediated excision of STEMCCA™ genes from iPS cells, derivation and rescue of new and existing ES cell lines using RESGRO™ Culture Medium and ESGRO Complete™ system.

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