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Merck
CN

SCR023

Sigma-Aldrich

Endothelial Cell Characterization Kit

Endothelial cell characterization kits enable researchers to characterize cultured endothelial cells in both a constitutive & activated state using basic ICC techniques.

别名:

Characterization Kit, Endothelial Cell Kit, Kit for Endothelial Characterization

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UNSPSC代码:
12352207
eCl@ss:
32161000
NACRES:
NA.84

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质量水平

100

种属反应性

human

制造商/商品名称

Chemicon®

技术

cell culture | stem cell: suitable
immunocytochemistry: suitable

输入

sample type: mouse embryonic stem cell(s)
sample type neural stem cell(s)
sample type hematopoietic stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)
sample type: human embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type mesenchymal stem cell(s)
sample type cardiac stem cell(s)

运输

wet ice

一般描述

Endothelial Cell Characterization Kit

Antibodies for the immunocytochemical characterization of endothelial cells.
Endothelial cells form the inner lining of blood vessels and serve as the cellular interface between the circulating blood and the vessel wall. Endothelial cells are involved in many aspects of vascular biology, including inflammation, angiogenesis, atherosclerosis, blood clotting, vasoconstriction and vasodilation. Chemicon′s endothelial cell characterization kit enables researchers to characterize cultured endothelial cells in both a constitutive and activated state using basic immunocytochemistry techniques.

Endothelial cells express the following markers constitutively. For optimal results, the following antibody dilutions are recommended for immunocytochemistry:

* Mouse anti-Endothelial Cell (CD146): 1/500 dilution based on 1 mg/mL, final 2 ng/mL

* Rabbit anti-VWF: 1/5000 dilution based on 1 mg/mL, final 0.2 ng/mL

* Mouse anti-CD31: 1/50 dilution based on 0.1 mg/mL, final 20 ng/mL

The following antibodies have been shown to stain cultured HUVEC after 4-6hr of TNF-alpha (100ng/ml) induction at the following dilutions:

* Mouse anti-ICAM-1: 1/500 dilution based on 1 mg/mL, final 2 ng/mL

* Mouse anti-VCAM-1: 1/500 dilution based on 1 mg/mL, final 2 ng/Ml

应用

Research Category
Stem Cell Research

制备说明

Antibodies to ICAM-1, VCAM-1, von Willibrand Factor (vWF), and Endothelial Cells (CD146) are to be stored at 2 - 8°C (SCR023 - Part 1). Antibodies to CD31, Mouse IgG, and Rabbit IgG are to be stored at -20°C (SCR023 - Part 2).

其他说明

50 μg of Endothelial Cell (CD146) monoclonal (Part No. 2003789)

50 μg of CD31 monoclonal (Part No. 2003788)

10 μg of von Willibrand Factor (vWF) polyclonal (Part No. 2003787)

50 μg of VCAM-1 monoclonal (Part No.MAB2511-50UG)

50 μg of ICAM-1 monoclonal (Part No. 2003785)

100 μg of Mouse control IgG (Part No. PP54-100UG)

100 μg of Rabbit control IgG (Part No. PP64-100UG)

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

10 - Combustible liquids

法规信息

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Human molecular genetics, 20(2), 235-244 (2010-10-16)
Human leukocyte antigen (HLA)-E is a non-classical major histocompatibility complex class I (Ib) molecule, which plays an important role in immunosuppression. In this study, we investigated the immunomodulating effect of HLA-E in a xenogeneic system, using human placental artery-derived endothelial
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