质量水平
种属反应性(根据同源性预测)
mammals
制造商/商品名称
Chemicon®
技术
cell culture | stem cell: suitable
输入
sample type: human embryonic stem cell(s)
sample type cardiac stem cell(s)
sample type neural stem cell(s)
sample type hematopoietic stem cell(s)
sample type epithelial cells
sample type mesenchymal stem cell(s)
sample type pancreatic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
一般描述
Alkaline phosphatase (ALP) is a hydrolase enzyme responsible for dephosphorylating molecules such as nucleotides, proteins, and alkaloids under alkaline conditions. The enzyme is present within all tissues of the body but is elevated in cells of the liver, kidney, bone, placenta, embryo and under specific disease states. Under alkaline conditions (pH>10), ALP can catalyze the hydrolysis of p-nitrophenylphosphate (p-NPP) into phosphate and p-nitrophenol, a yellow colored by-product of the catalytic reaction. The amount of p-nitrophenol produced is proportional to the amount of alkaline phosphatase present within the reaction. The amount of ALP can thus be reliably quantified by reading the amount of p-nitrophenol amassed after the catalytic reaction at 405 nm on a spectrophotometer.
应用
Stem Cell Research
制备说明
其他说明
p-NPP Buffer: (Part No. ES011-10ML) 10 mL
Reaction Stop Solution: (Part No.CS200653) 5 mL
1X Wash Solution: (Part No. CS200652) 2 X 125 mL
Recombinant Alkaline Phosphatase Standard: (Part No. CS200654) 0.5 μg (10 μg/mL)
法律信息
免责声明
警示用语:
Danger
危险分类
Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Corr. 1B
储存分类代码
8A - Combustible corrosive hazardous materials
法规信息
相关内容
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As the focus of stem cell research undergoes a transition from animal to human models, researchers are in critical need of validated products to support the isolation, maintenance, differentiation, and characterization of human stem cells. While many reagents designed for rodent systems can be applied to human stem cell studies, they are not truly optimized for robust human stem cell culture or analysis. This is why human stem cell researchers have always trusted EMD Millipore, the first provider of commercially available human embryonic stem cells and human neural stem cell lines, to accelerate their research. All of our human stem cell systems are extensively tested in defined media culture, and differentiated progeny are comprehensively characterized with highly validated antibodies and detection reagents.
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