跳转至内容
Merck
CN

SCR066

Quantitative Alkaline Phosphatase ES Characterization Kit

This Quantitative Alkaline Phosphatase Embryonic Stem (ES) Characterization Kit easily quantifies the amount of alkaline phosphatase present within their embryonic stem cell cultures using a convenient 96-well colorimetric enzymatic assay.

登录 查看公司和协议定价

选择尺寸


关于此项目

UNSPSC代码:
12352207
eCl@ss:
32161000
NACRES:
NA.81
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

质量水平

种属反应性(根据同源性预测)

mammals

制造商/商品名称

Chemicon®

技术

cell culture | stem cell: suitable

输入

sample type: human embryonic stem cell(s)
sample type cardiac stem cell(s)
sample type neural stem cell(s)
sample type hematopoietic stem cell(s)
sample type epithelial cells
sample type mesenchymal stem cell(s)
sample type pancreatic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

一般描述

Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass (ICM) of preimplantation embryos and are capable of unlimited, undifferentiated proliferation in vitro under appropriate cell culture conditions. Undifferentiated embryonic stem cells are characterized by high expression levels of stage-specific embryonic antigens (SSEA-1, 3 and 4), tumor rejection antigens (TRA-1-60 and TRA-1-81), OCT-4 and alkaline phosphatase. These undifferentiated markers are downregulated upon spontaneous or induced differentiation.

Alkaline phosphatase (ALP) is a hydrolase enzyme responsible for dephosphorylating molecules such as nucleotides, proteins, and alkaloids under alkaline conditions. The enzyme is present within all tissues of the body but is elevated in cells of the liver, kidney, bone, placenta, embryo and under specific disease states. Under alkaline conditions (pH>10), ALP can catalyze the hydrolysis of p-nitrophenylphosphate (p-NPP) into phosphate and p-nitrophenol, a yellow colored by-product of the catalytic reaction. The amount of p-nitrophenol produced is proportional to the amount of alkaline phosphatase present within the reaction. The amount of ALP can thus be reliably quantified by reading the amount of p-nitrophenol amassed after the catalytic reaction at 405 nm on a spectrophotometer.
Millipore’s Quantitative Alkaline Phosphatase ES Characterization Kit allows researchers to easily quantify the amount of alkaline phosphatase present within their embryonic stem cell cultures using a convenient 96-well colorimetric enzymatic assay. This kit can be used in conjunction with Millipore’s Alkaline Phosphatase Detection kit (Cat. No. SCR004) to fully characterize the levels of alkaline phosphatase present within an embryonic stem cell culture.

应用

Research Category
Stem Cell Research
This Quantitative Alkaline Phosphatase Embryonic Stem (ES) Characterization Kit easily quantifies the amount of alkaline phosphatase present within their embryonic stem cell cultures using a convenient 96-well colorimetric enzymatic assay.

制备说明

All reagents are to be stored at 2∘ to 8∘C until expiration date. p-NPP substrate concentrate and p-NPP buffer are light sensitive and should not be exposed to light. Reaction stop solution contains 1N sodium hydroxide and is corrosive. Avoid skin contact and inhalation.

其他说明

p-NPP Substrate Concentrate (50X): (Part No. ES008-200UL) 200 μL

p-NPP Buffer: (Part No. ES011-10ML) 10 mL

Reaction Stop Solution: (Part No.CS200653) 5 mL

1X Wash Solution: (Part No. CS200652) 2 X 125 mL

Recombinant Alkaline Phosphatase Standard: (Part No. CS200654) 0.5 μg (10 μg/mL)

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

象形图

Health hazardCorrosion

警示用语:

Danger

危险分类

Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Corr. 1B

储存分类代码

8A - Combustible corrosive hazardous materials

法规信息

新产品
此项目有

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Ho-Chang Jeong et al.
Stem cells (Dayton, Ohio), 35(9), 2037-2049 (2017-05-26)
Basic fibroblast growth factor (bFGF) supplementation is critical to maintain the pluripotency of human pluripotent stem cells (hPSCs) through activation of PI3K/AKT, rather than MEK/ERK pathway. Thus, elaborate molecular mechanisms that preserve PI3K/AKT signaling upon bFGF stimulation may exist in
Jian Fang Liu et al.
Tissue engineering. Part A, 17(17-18), 2343-2357 (2011-05-10)
Differentiation of embryoid bodies (EBs) into particular cell lineages has been extensively studied. There is an increasing interest in the effect of soft hydrogel scaffolds on the behavior of EBs, such as the initial adhesion, dynamic morphology change, and differentiation.
Fei Deng et al.
Molecular vision, 18, 2871-2881 (2012-12-13)
This study aimed to develop a feasible and efficient method for generating embryonic stem cell (ESC)-like induced pluripotent stem (iPS) cells from human Tenon's capsule fibroblasts (HTFs) through the expression of a defined set of transcription factors, which will have
Narimon Honarpour et al.
Molecular & cellular proteomics : MCP, 13(3), 780-791 (2014-01-07)
The programmed formation of specific tissues from embryonic stem cells is a major goal of regenerative medicine. To identify points of intervention in cardiac tissue formation, we performed an siRNA screen in murine embryonic stem cells to identify ubiquitin system
Mehdi Shafa et al.
Frontiers in medicine, 5, 69-69 (2018-03-31)
The discovery of reprogramming and generation of human-induced pluripotent stem cells (iPSCs) has revolutionized the field of regenerative medicine and opened new opportunities in cell replacement therapies. While generation of iPSCs represents a significant breakthrough, the clinical relevance of iPSCs

相关内容

We have collaborated with Plasticell Ltd. to develop OsteoMAX-XF™, a novel, serum-free, xeno-free, differentiation medium for the generation of osteoblasts from human MSCs. This medium may be particularly suitable for drug discovery and clinical development. By screening roughly 3,500 combinations of serum and xeno-free media, we discovered a remarkably efficient, fully defined, reproducible medium to promote the differentiation of hMSCs into osteoblasts, offering an excellent, cost effective method to produce large amounts of human bone cells for multiple applications.

As the focus of stem cell research undergoes a transition from animal to human models, researchers are in critical need of validated products to support the isolation, maintenance, differentiation, and characterization of human stem cells. While many reagents designed for rodent systems can be applied to human stem cell studies, they are not truly optimized for robust human stem cell culture or analysis. This is why human stem cell researchers have always trusted EMD Millipore, the first provider of commercially available human embryonic stem cells and human neural stem cell lines, to accelerate their research. All of our human stem cell systems are extensively tested in defined media culture, and differentiated progeny are comprehensively characterized with highly validated antibodies and detection reagents.

查看所有结果

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持