SCR103
I型胶原酶
The collagenase type I (from Clostridium histolyticum) is a crude collagenase preparation that can be used for the isolation of primary cells or for tissue dissociation by enzymatic means.
别名:
Collagenase Type I from Clostridium histolyticum, Tissue dissociation
质量水平
表单
powder
制造商/商品名称
Chemicon®
技术
cell culture | stem cell: suitable
输入
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
运输
wet ice
一般描述
I 型胶原酶（来自溶组织梭状芽胞杆菌）是一种粗制的胶原酶制剂，可用于分离原代细胞或通过酶促方法进行组织分离。该制剂还可能含有酪蛋白酶、梭菌蛋白酶和胰蛋白酶活性。该制剂可用于分离培养中的细胞，并已成功用于传代用 Millipore 的 HEScGROTM 无动物成分培养基培养的人胚胎干细胞。
包装
1 小瓶包含 250mg 冻干粉
外形
I 型胶原酶以透析的冻干粉形式提供。
制备说明
I 型胶原酶应以粉末形式在 2-8oC 下保存，直到可以使用为止。可以将 I 型胶原酶溶液等分并保存在 -20oC，但不应反复冻融。
分析说明
通过改良的 Mandl（1964）来测定每批 I 型胶原酶，并使用 Moore and Stein（1948）的比色茚三酮法测定胶原蛋白分解的程度。然后测试每批在 Millipore 的 HEScGRO hES 细胞培养基中培养的人胚胎干细胞成功传代的情况。经过五次传代后，对细胞进行合适的标志物表达和形态学表征。
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
仅供研究使用。在法国，在用于科研目的时（包括进口和出口活动（《公共健康法》第L 1211-1条，第2节））该产品为管制品。购买者（即最终用户）需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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