BioTracker^™ Green Exosome Membrane Labeling Kit

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This live cell probe fluoresces in the green spectral range when it binds to the lipid bilayer in exosome membranes.

Exosomes are a form of extracellular vesicle (EV), which can contribute to intercellular communication. To elucidate such communication, labeling techniques based on fluorescent dyes have been developed. Fluorescent dyes that label the cellular membrane are commonly used for exosome labeling because the lipid bilayer in exosomes is a suitable labeling target.

The BioTracker^™ exosome membrane labeling probe series can be used for staining of the membranes of purified exosomes, which in turn permits imaging of labeled exosomes taken up by cells.SCT265 does not cause aggregation and has little effect on the properties of exosomes, permitting accurate observation of exosome dynamics.BioTracker^™ exosome membrane labeling probes overcome issues with existing labeling dyes, including:1) self-particle formation2) an increase in size of the exosomes after labelingThree different fluorescence colors---green (SCT265), red (SCT266), and far red (SCT267)--- enable application in multiplexing experiments, such as observation of exosome localization in organelles.Add 10 uL DMSO to tube of green, red, or far red exosome membrane dye to create stock solution. SPECTRAL PROPERTIES:Ex 488nm/Em 490-540nmReferences:Kawata R, Oda S, Koya Y, Kajiyama H, Yokoi T. 2020. Macrophage-derived extracellular vesicles regulate concanavalin A-induced hepatitis by suppressing macrophage cytokine production. Toxicology. 443:152544.

This live culture probe for the detection of exosomes labels the lipid bilayers of exosomal membranes, enabling observation of the uptake of exosomes by cells. Exosomes should be purified before labeling.

Store BioTracker^™ Green Exosome Membrane Labeling Kit at 2 - 8 °C, protected from light.After resuspending in DMSO, store stock solution at -20 °C, protected from light.

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany