BioTracker^™ Red pH-Independent Autophagy Live Cell Probe

BioTracker Red pH-Independent Autophagy Live Cell Probe detects autophagosomes and autolysosomes. The presumptive mechanism involves incorporation of the probe via structural features into the autophagosome during double-membrane formation. Upon incorporation, the probe emits fluorescence under hydrophobic conditions. The utility of the probe is conferred by molecular properties that include cell permeability, does not require transfection, and enables live cell detection with fluorescence microscopy or other fluorescence capture instruments.

The small fluorescent molecule SCT277 is used to detect autophagosomes and autolysosomes. The presumed mechanism is that the dye is incorporated into the autophagosome during double-membrane formation via structural features, and then emits fluorescence under hydrophobic conditions. The probe emits in the far red range.SPECTRAL CHARACTERISTICS: Excitation λ: 530 nmEmission λ: 720 nm

Fluorescence microscopyFlow cytometry

Store at -20 °C, protected from light.

SCT277 detects autophagosomes and autolysosomes by the likely mechanism of incorporation into the autophagosome during double membrane formation, and emits red fluorescence under hydrophobic conditions. SCT277 is cell permeable, does not require transfection, and enables live cell imaging with fluorescence microscopy and quantitative assay by flow cytometry.Add 50 uL DMSO to tube of SCT277 (5 nmol) and pipet to dissolve to make a 0.1 mM stock solution. Dilute with culture medium to 0.1 - 0.5 uM to make working solution.

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

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