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Merck
CN

SNAP2MINI

SNAP id® 2.0 Protein Detection System-Mini

7.5 x 8.4 cm, Unique vacuum-driven technology & a built-in flow distributor actively drive reagents through the membrane

别名:

蛋白质检测试剂盒

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eCl@ss:
42029053
UNSPSC Code:
41116010
NACRES:
NB.22
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产品名称

SNAP id® 2.0蛋白检测加速系统-Mini (7.5 x 8.4 cm), Developed to meet the needs of our Western blotting customers, the SNAP i.d. 2.0 system produces blots of a very high quality. Unique vacuum-driven technology & a built-in flow distributor actively drive reagents through the membrane..

manufacturer/tradename

SNAP id®

technique(s)

western blot: suitable

compatibility

for use with Commercially available blocking reagents, for use with Luminata Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with blØk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01), for use with blØk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01), for use with blØk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001), for use with commercially available detection reagents

detection method

chemiluminescent, colorimetric, fluorometric

shipped in

ambient

storage temp.

room temp

General description

最新研发的SNAP id 2.0蛋白检测系统是SNAP i.d.的第二代,用于检测蛋白质印迹膜上的免疫反应性蛋白。独创性真空驱动机制,大大减少免疫检测时间。传统的蛋白质印迹耗时4-24小时,SNAP i.d. 2.0加速系统缩短至30分钟,同时不造成质量和信号损失。SNAP id 2.0加速系统用于蛋白转移到膜后的全部免疫检测步骤,包括封闭、洗涤、一抗二抗孵育。SNAP id 2.0迷你膜夹片可容纳7.5 × 8.4 cm大小的印迹膜。

Application

SNAP id 2.0蛋白检测系统-迷你型(7.5 x 8.4 cm)用于蛋白质印迹分析(western blot)。

Features and Benefits

SNAP id 2.0蛋白检测系统的特点:
  • 检测基座上是2个独立控制的真空驱动单元,最多可同时处理4张印迹膜
  • 可拆卸印迹框有三种尺寸:MultiBlot、Mini和Midi,可容纳不同大小的膜
  • 适合MultiBlot、Mini和Midi印迹框的一次性膜夹片
  • SNAP id第一代系统需要的印迹隔片已经融入新一代的膜夹片
  • 可选择延长印迹处理或脱机处理:印迹框带盖,可从基座取下,进行延长孵育(1小时至过夜)、振荡器孵育或4 °C孵育
  • 可叠放印迹框可同时处理多张膜。30分钟即可完成免疫检测反应,获得整张均匀的膜信号
  • 使用SNAP id 2.0抗体收集盘,抗体回收率高于80%
  • 兼容硝酸纤维素膜和聚偏氟乙烯(PVDF)膜
  • 适用大部分缓冲液和可视化方法,例如,化学发光(chemiluminescence)、荧光法、比色法

Packaging

该系统包括SNAP id® 2.0检测基座、2个迷你印迹框、2个抗体收集盘、真空管、印迹滚筒&垫板、2个液槽、简要操作说明书。

Other Notes

SNAP id® 2.0蛋白检测系统仅供研究使用。不可用于诊断程序。
替代品:WBAVDBASE

Legal Information

SNAP id is a registered trademark of Merck KGaA, Darmstadt, Germany

法规信息

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Nutritional neuroscience, 22(7), 499-512 (2017-12-19)
Previous studies have shown that dietary prebiotics have the potential to improve memory, alter social behavior, and reduce anxiety-like behaviors in rodents. The present research sought to expand upon such results and describe the effects of feeding prebiotics early in

商品

Understand the importance of using high-quality water in protein electrophoresis and Western blotting to improve assay sensitivity and accuracy.

实验方案

The SNAP i.d. 2.0 Protein Detection System is the second generation of the SNAP i.d. method for detecting immunoreactive proteins on Western blots.

相关内容

我们明白。西部片的污点可能会让人头疼,我们当然也有过这样的经历。因此,请与我们分享您的丑陋污点,并获得一份惊喜!虽然我们不能告诉你是什么--那会破坏惊喜的气氛--但它会激发实验室的创造力。

We get it. Westerns can be a pain in the blot, and we’ve certainly made our share. So, share with us your ugly blots and receive a surprise! And while we can’t tell you what it is – that would spoil the surprise - except that it will spark creativity in the lab.

Antibody reuse for Western blotting is a common practice for many researchers. While many antibodies lose potency with time or degrade even faster due to improper storage conditions, it is important to recognize the potential value of recovering the primary antibody for possible reuse in some experiments. The SNAP i.d.® 2.0 system is not only able to reduce the immunodetection processing time, but its flexibility lets you combine conditions used in the standard immunodetection protocol and also allows the collection of antibody for future reuse. Here, we compare the antibody recovery and reuse in the standard immunodetection protocol with the antibody recovery and reuse in SNAP i.d.® system using the extended protocol and the original SNAP i.d.® protocol.

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