ST1202
抗-PGC-1α 小鼠mAb (4C1.3)
liquid, clone 4C1.3, Calbiochem®
别名:
Anti-Peroxisome Proliferator-Activated Receptor- γ Coactivator 1 α
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
4C1.3, monoclonal
Species reactivity:
mouse, human, rat
Application:
—
Citations:
154
isotype
IgG
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
4C1.3, monoclonal
form
liquid
does not contain
preservative
species reactivity
mouse, human, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(Immunoblotting (1 µg/mL)
Immunocytochemistry (5-10 µg/mL)
Immunoprecipitation (10 µg/mL)
Paraffin Sections (whole mount) )
storage temp.
−70°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PPARGC1A(10891)
General description
PGC-1α是PPARα、PPARγ和其他转录因子的共激活因子，可调节棕色脂肪组织中适应性生热、肝/肾糖异生和肌肉纤维类型转换的转录程序。全长蛋白质为113 kDa，通过冷暴露在棕色脂肪组织中诱导，通过禁食在肝脏和肾脏中诱导，并通过运动在骨骼肌中诱导。全长基因的可变剪接产生270aa的N末端剪接变体，其在〜38kDa处迁移并由诱导全长蛋白质表达的相同生理信号诱导。N末端PGC-1α剪接变体也受到翻译后修饰的影响，从而改变其迁移和表观分子量。
蛋白G纯化的小鼠单克隆抗体。识别内源形式的PGC-1α，包括~113 kDa PGC-1α蛋白和~38 kDa剪接变体。
识别棕色脂肪组织、肝脏和肾脏中的内源形式的PGC-1α，包括~113 kDa PGC-1α蛋白和~38 kDa剪接变体。
该抗PGC-1α小鼠单克隆抗体（4C1.3）经验证可用于免疫印迹、免疫细胞化学、免疫沉淀、石蜡切片以检测PGC-1α。
Immunogen
小鼠
由小鼠PGC-1的1-120位氨基酸组成的重组蛋白α
Application
免疫印迹（1 µg/mL）
免疫细胞化学（5-10 µg/mL）
免疫沉淀（10 µg/mL）
石蜡切片（整个安装）（请参阅应用参考文献）
免疫细胞化学（5-10 µg/mL）
免疫沉淀（10 µg/mL）
石蜡切片（整个安装）（请参阅应用参考文献）
Physical form
1小瓶100 µg抗体溶于50 mM PBS中，参见批次特定浓度的小瓶。一小瓶NT-PGC-1α阳性对照，在含有10 mM &-巯基乙醇和1%SDS的RIPA缓冲液中以25 µl全细胞提取物的形式提供；每个泳道上样10 µl；在SDS-PAGE上样前加入样品缓冲液。
Preparation Note
初次解冻后，分装冻存（-70°C）。
Other Notes
Lai, L., et al. 2008.Genes Dev.14, 1948.
Rodgers, J.T., et al. 2008.FEBS Lett.582, 46.
Mazzucotelli, A., et al. 2007.Diabetes 10, 2467.
Nemoto, S., et al. 2005.J. Biol. Chem. 16, 16456.
Rodgers, J.T., et al. 2008.FEBS Lett.582, 46.
Mazzucotelli, A., et al. 2007.Diabetes 10, 2467.
Nemoto, S., et al. 2005.J. Biol. Chem. 16, 16456.
内源性PGC-1α主要存在于细胞核中，NT-PGC-1α存在于胞质和核部分中。在单个系统中，应对抗体进行滴定以获得最佳结果。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性：多个毒性值，请参阅MSDS（O）
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存储类别
10 - Combustible liquids
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