biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
3F2-1F3, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA (see coments)
Immunoblotting (1-5 µg/mL)
Immunocytochemistry (10 µg/mL)
Paraffin Sections (10 µg/mL))
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... FASN(2194)
General description
Anti-FASN, mouse monoclonal, clone 3F2-1F3, recognizes FASN protein in 293 and HeLa cells and in human breast carcinoma tissue. It is validated for ELISA, WB, ICC, and IHC (paraffin sections).
Purified mouse monoclonal antibody. Recognizes the FASN protein.
Recognizes the FASN protein in 293 and HeLa cells and human breast carcinoma tissue.
Immunogen
Full-length, recombinant human FASN (aa 1-440) expressed as GST fusion protein
Application



ELISA (see coments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (10 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
293 cells, HeLa cells, Human breast carcinoma tissue
293 cells, HeLa cells, Human breast carcinoma tissue
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Kristiansen, G., et al. 2010. Br. J. Cancer102, 1736.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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Boubacar Benziane et al.
Journal of lipid research, 58(12), 2324-2333 (2017-10-27)
Diacylglycerol kinases (DGKs) regulate the balance between diacylglycerol (DAG) and phosphatidic acid. DGKζ is highly abundant in skeletal muscle and induces fiber hypertrophy. We hypothesized that DGKζ influences functional and metabolic adaptations in skeletal muscle and whole-body fuel utilization. DAG
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| 货号 | GTIN |
|---|---|
| ST1549-50UG | 04055977224252 |