biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
2F11, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA
Immunoblotting (1-5 µg/mL)
Paraffin Sections (3 µg/mL))
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... FGF21(26291)
General description
Anti-FGF21, mouse monoclonal, clone 2F11, recognizes the FGF21 protein in human tonsil tissue. It is validated for use in ELISA, Western blotting, and Immunohistochemistry (paraffin sections).
Purified mouse monoclonal antibody. Recgonizes the FGF21 protein.
Recgonizes the FGF21 protein in human tonsil tissue.
Immunogen
Full-length, recombinant human FGF21 (aa 30-210), expressed as a GST fusion protein
Application


ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (3 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Human tonsil tissue
Human tonsil tissue
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Junjie Xiao et al.
Journal of cellular and molecular medicine, 20(2), 204-216 (2015-12-10)
Non-alcoholic fatty liver disease (NAFLD) is associated with obesity and lifestyle, while exercise is beneficial for NAFLD. Dysregulated microRNAs (miRs) control the pathogenesis of NAFLD. However, whether exercise could prevent NAFLD via targeting microRNA is unknown. In this study, normal
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