ST1629
Anti-GNA13 Mouse mAb (6F6-B5)
liquid, clone 6F6-B5, Calbiochem®
别名:
Anti-G Protein α13, Anti-Guanine Nucleotide Binding Protein α13, Anti-Guanine Nucleotide Binding Protein α13, Anti-G Protein α13
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
6F6-B5, monoclonal
Species reactivity:
human
Application:
—
Citations:
3
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
6F6-B5, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA
Immunoblotting (1-5 µg/mL))
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... GNA13(10672)
General description
Anti-GNA13, mouse monoclonal, clone 6F6-B5, recognizes the ~40-45 kDa GNA13 protein in HepG2 cells. It is validated for use in ELISA and Western blotting.
Purified mouse monoclonal antibody. Recognizes the ~40-45 kDa GNA13 protein.
Recognizes the ~40-45 kDa GNA13 protein in HepG2 cells.
Immunogen
Full-length, recombinant, human GNA13 (aa 1-378), expressed as a GST fusion protein
Application
ELISA (see comments)
Immunoblotting (1-5 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Negative Control
293T cells
293T cells
Positive Control
HepG2 cells
HepG2 cells
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
MicroRNA-31 controls G protein alpha-13 (GNA13) expression and cell invasion in breast cancer cells.
Suhail Ahmed Kabeer Rasheed et al.
Molecular cancer, 14, 67-67 (2015-04-19)
Gα13 (GNA13) is the α subunit of a heterotrimeric G protein that mediates signaling through specific G protein-coupled receptors (GPCRs). Our recent study showed that control of GNA13 expression by specific microRNAs (miRNAs or miRs) is important for prostate cancer
Suhail Ahmed Kabeer Rasheed et al.
The Journal of biological chemistry, 288(11), 7986-7995 (2013-01-19)
G protein-coupled receptors (GPCRs) and their ligands have been implicated in progression and metastasis of several cancers. GPCRs signal through heterotrimeric G proteins, and among the different types of G proteins, GNA12/13 have been most closely linked to tumor progression.
Suhail Ahmed Kabeer Rasheed et al.
Oncogene, 37(10), 1340-1353 (2017-12-20)
Treatment failure in solid tumors occurs due to the survival of specific subpopulations of cells that possess tumor-initiating (TIC) phenotypes. Studies have implicated G protein-coupled-receptors (GPCRs) in cancer progression and the acquisition of TIC phenotypes. Many of the implicated GPCRs
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