biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
1G7, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA (see coments)
Immunoblotting (1-5 µg/mL)
Immunocytochemistry (10 µg/mL))
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... AFP(174)
General description
Anti-AFP, mouse monoclonal, clone 1G7, recognizes ~70-75 kDa AFP protein in HepG2 cells. It is validated for ELISA, Western blotting, and immunocytochemistry.
Purified mouse monoclonal antibody. Recognizes the ~70-75 kDa AFP protein.
Recognizes the ~70-75 kDa AFP protein in HepG2 cells.
Immunogen
A recombinant polypeptide corresponding to amino acids of 500-610 human AFP, expressed as a GST fusion protein
Application
ELISA (see coments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Physical form
In PBS, pH 7.4.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Negative Control
293T cells
293T cells
Positive Control
HepG2 cells
HepG2 cells
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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