biological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
2G11-1C11, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA (see coments)
Immunoblotting (1-4 µg/mL)
Immunocytochemistry (10 µg/mL))
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... NDE1(54820)
General description
Recognizes the ~40-45 kDa NDE1 protein in HeLa cells.
Purified mouse monoclonal antibody. Recognizes the ~40-45 kDa NDE1 protein.
This Anti-NDE1 Mouse mAb (2G11-1C11) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of NDE1.
Immunogen
Full-length, recombinant, human NDE1 (aa 1-336), expressed as a GST fusion protein
Application


ELISA (see coments)
Immunoblotting (1-4 g/ml)
Immunocytochemistry (10 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
HeLa cells
HeLa cells
Other Notes
Bradshaw, N.J., et al. 2009. Neurosci. Lett.449, 228.
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable