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Merck
CN

UFC201024

Amicon® Ultra离心超滤管，10 kDa MWCO

greener alternative

sample volume 2 mL, regenerated cellulose membrane, MWCO 10 kDa

别名:

离心浓缩器，10 kDa MWCO，2 mL样品体积

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关于此项目

UNSPSC Code:
41104916
NACRES:
NB.24
eCl@ss:
36100101

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产品名称

Amicon® Ultra离心超滤管，10 kDa MWCO, sample volume 2 mL, regenerated cellulose membrane, MWCO 10 kDa

material

polypropylene tube (for collection)
regenerated cellulose membrane
styrene-butadiene housing

sterility

non-sterile

product line

Amicon®

packaging

pkg of 24 pkg

manufacturer/tradename

Amicon® Ultra

sustainability

Greener Alternative Product

parameter

2 mL sample volume

technique(s)

protein extraction: suitable
protein purification: suitable

L

119.7 mm

diam.

15.9 mm

filtration area

1 cm2

volume

2 mL

working volume

≤2 mL
2 mL

pore size

10 kDa MWCO

Quality Level

400

greener alternative category

Re-engineered

shipped in

ambient

相关类别

Other Notes

替代品：4205
Amicon® Ultra-2装置随附两根管。在操作过程中，一根管用来回收滤液；另一根管在浓缩过程中用来封盖设备，随后回收浓缩样本。Amicon Ultra-2离心过滤装置仅供研究使用，不能用于诊断程序。

Application

  • 采用10,000 Dalton分子量截止超滤®膜进行超滤。
  • 含抗原、抗体、酶、核酸或微生物的生物样品的浓度。
  • 纯化在组织培养提取物或细胞裂解液中发现的大分子成分。
  • 引物去除、PCR清理、从反应混合物中去除连接物或大分子标记，以及在HPLC之前去除蛋白质。
  • 脱盐，缓冲液更换和蛋白质透析。
  • 体积排阻和蛋白质分离。
Amicon® Ultra-2离心过滤装置已用于：
  • 在细胞外囊泡(EV)分离前减少大量生物液体或浓缩细胞外囊泡
  • 浓缩含抗原、抗体、酶、核酸(DNA/RNA样品，单链或双链)、微生物、柱洗脱物和纯化样品的生物样品
  • 在高效液相色谱(HPLC)分析之前，对组织培养提取物和细胞裂解液中发现的大分子成分进行纯化，从反应混合物中去除引物、连接物或分子标记，去除蛋白质
  • 脱盐、缓冲交换或过滤

Features and Benefits

  • Amicon® Ultra 2 ml 10K 24支包装
  • 可浓缩2 mL至15-70 μl
  • 98%的样品回收率
  • 快速的处理时间-10至30分钟的离心时间
  • 工程化永停点
  • 反向离心功能可提供一致的回收率

  • 使用10 kda NMWCO的Ultracel®再生纤维素膜提供大于90%的保留回收率
  • 2 mL浓缩至15-70 μL
  • 98％的样品回收率
  • 快速处理时间：10至30分钟旋转时间
  • 工程化永停点
  • 反向旋转能力提供一致的回收率

General description

Amicon® Ultra-2离心过滤装置提供快速超滤。它具有从稀释的和复杂的样品基质中回收高浓度因子和轻松浓缩的潜力。Amicon® Ultra-2设备未经消毒，仅供一次使用。
我们竭诚为您带来经过重新设计的绿色替代产品，实现可持续性更高的研究方案。 内部过滤装置采用按ISCC质量平衡分配的生物循环材料制成。点击此处以获取更多信息。

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

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Lulai Xu et al.
Vaccine, 38(7), 1690-1699 (2020-01-16)
Newcastle disease virus (NDV) has been used as a vector in the development of vaccines and gene delivery. In the present study, we generated the thermostable recombinant NDV (rNDV) expressing the different forms of hemagglutinin (HA) of highly pathogenic avian
Bao Quoc Tran et al.
Data in brief, 6, 68-76 (2016-01-23)
A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated
Keerthie Dissanayake et al.
Theriogenology, 149, 104-116 (2020-04-08)
Extracellular vesicles (EVs) are membrane-bound biological nanoparticles (NPs) and have gained wide attention as potential biomarkers. We aimed to isolate and characterize EVs from media conditioned by individually cultured preimplantation bovine embryos and to assess their relationship with embryo quality.
Glenn Vergauwen et al.
Scientific reports, 7(1), 2704-2704 (2017-06-04)
Identification and validation of extracellular vesicle (EV)-associated biomarkers requires robust isolation and characterization protocols. We assessed the impact of some commonly implemented pre-analytical, analytical and post-analytical variables in EV research. Centrifugal filters with different membrane types and pore sizes are
Jasjeet Kaur et al.
Virus genes, 56(4), 480-497 (2020-05-06)
Staphylococcus aureus is one of the most dreadful infectious agents, responsible for high mortality and morbidity in both humans and animals. The increased prevalence of multidrug-resistant (MDR) Staphylococcus aureus strains has limited the number of available treatment options, which calls

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蛋白样品超滤

本页内容讨论了使用超滤膜进行蛋白样品制备的原理和优势。应用包括溶剂置换、蛋白样品浓缩或纯化。

实验方案

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