选择尺寸
关于此项目
Quality Level
material
polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg, pkg of 500 pkg, pkg of 8 pkg, pkg of 96 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Greener Alternative Product
parameter
0.5 mL sample volume
technique(s)
protein extraction: suitable, protein purification: suitable
L
4.99 cm (1.96 in.)
diam.
10.8 mm
filtration area
1 cm2
volume
0.5 mL
working volume
≤500 μL , 0.5 mL
pore size
10 kDa MWCO
greener alternative category
shipped in
ambient
General description
垂直设计和可用滤膜表面积可实现快速样品处理、高样品回收率(通常超过稀释起始溶液的90%)以及30倍浓度。垂直设计可最大限度地减少溶解物极化及随后的滤膜污染,并且过滤装置中的物理停止可防止旋转至干燥及潜在样品损失。收集滤液后,可通过反向旋转快捷操作高效回收浓缩样品(保留品种)。
Amicon® Ultra-0.5装置未经灭菌处理,仅供一次性使用。Amicon® Ultra 0.5 mL过滤器的垂直滤膜设计和反向旋转功能可在更短的旋转时间内实现较高的性能。
Application
- 采用10,000 Dalton分子量截留Ultracel®滤膜超滤
- 浓缩含抗原、抗体、酶、核酸或微生物的生物样品
- HPLC之前,纯化在组织培养基提取物或细胞裂解物中发现的高分子组分、去除引物、连接子或标记反应混合物中的高分子并去除蛋白质
- 脱盐、缓冲液更换和蛋白透析等
Features and Benefits
- Ultracel®再生纤维素滤膜配有10 kDa NMWCO,其保留物回收率超过90%
- 浓缩500 μL至15 μL
- 浓缩系数25至30倍
- 样品回收率高(稀释起始溶液超过90%)
- 处理时间快:10至30分钟
- 反向旋转功能,确保一致的回收率
Physical form
Legal Information
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Explore all of our products under Amicon® Ultra过滤器,10 kDa MWCO
实验方案
Detergents are frequently used to solubilize proteins and nucleic acids during purification, but the presence of detergents may interfere with downstream analyses.
Best practices for standardizing and reducing the Extracellular Vesicle Preparation workflow, and eliminating contamination of EV preparations with dead-cell-derived vesicles.
If an antibody must be purified from a small sample volume (0.5-10 mL), then the Amicon® Pro Purification System is recommended.
商品
Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.
PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.
Commercially available protein labeling kits provide researchers with the flexibility to customize their own immunoassay detection panels.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| UFC501008 | 04053252663109 |
| UFC501096 | 08436037125768 |
| UFC501024 | 04053252465345 |
| UFC5010BK | 04053252397417 |