产品名称
Amicon® Ultra过滤器,10 kDa MWCO, sample volume 4 mL, regenerated cellulose membrane, MWCO 10 kDa
material
polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg
pkg of 8 pkg
pkg of 96 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Greener Alternative Product
parameter
4 mL sample volume
technique(s)
protein extraction: suitable
protein purification: suitable
L
124 mm (4.88 in.)
diam.
17.3 mm
filtration area
3 cm2
volume
4 mL
working volume
≤4 mL
4 mL
pore size
10 kDa MWCO
Quality Level
greener alternative category
shipped in
ambient
Application
- 采用10,000 Dalton分子量截止超滤®膜进行超滤。
- 含抗原、抗体、酶、核酸或微生物的生物样品的浓度。
- 纯化在组织培养提取物或细胞裂解液中发现的大分子成分,并在HPLC分析之前去除蛋白质。
- 从柱洗脱液稀释或预先纯化的蛋白质的浓度。
- 脱盐,缓冲液更换和蛋白质透析。
- 体积排阻和蛋白质分离。
Features and Benefits
- 使用10 kda NMWCO的Ultracel®再生纤维素膜提供大于90%的保留回收率。
- 垂直膜减少了超快旋转时间(快至5分钟)的浓度极化。
- 热封膜减少了下游的可提取物。
- 100%完整性测试,确保可靠性能。
- 半透明外壳和体积刻度便于样品监测。
- 直接移液管进样,省去了回收浓缩液的处理步骤。
- 80–100X高浓缩系数。
General description
垂直设计和有效的膜表面积提供了快速的样品处理,高样品回收率(通常大于90%的稀起始溶液),以及80倍浓度的处理能力。垂直设计最大限度地减少了溶质极化和随后的膜污染,过滤装置中的物理永停点可防止纺丝干燥和潜在的样品损失。
使用移液管从过滤装置的样品库中收集浓缩液,同时将超滤液收集在所提供的离心管中。该装置可在摆动斗中旋转,也可在定角转子中旋转。Amicon® Ultra 15 mL过滤器采用垂直膜设计和永停点,可在短旋转时间内提供最高回收率。
Legal Information
存储类别
10-13 - German Storage Class 10 to 13
商品
Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.
Filtration methods and membrane filter selection in nanoparticle purification and production.
实验方案
The human plasma proteome is a vast resource for biomarker discovery and investigation.
Best practices for standardizing and reducing the Extracellular Vesicle Preparation workflow, and eliminating contamination of EV preparations with dead-cell-derived vesicles.
Accurate measurement of specific proteins in urine is important for diagnosing and managing diseases.
Using a standard Amicon® Ultra centrifugal filter, a few rounds of diafiltration can efficiently remove unincorporated label from an antibody labeling reaction without diluting the sample.
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