Immobilon^® Classico Western HRP substrate

A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.

Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.

We get it. Westerns can be a pain in the blot, and we’ve certainly made our share. So, share with us your ugly blots and receive a surprise! And while we can’t tell you what it is – that would spoil the surprise - except that it will spark creativity in the lab.

我们明白。西部片的污点可能会让人头疼，我们当然也有过这样的经历。因此，请与我们分享您的丑陋污点，并获得一份惊喜！虽然我们不能告诉你是什么--那会破坏惊喜的气氛--但它会激发实验室的创造力。