产品名称
碱性磷酸酶重组，高活性, EIA Grade, from Pichia Pastoris
assay
≥95% (HPLC)
form
buffered aqueous solution (ready-to-use, 20 mg/ml)
specific activity
≥7000 units/mg protein (At 37 °C with pNPP and DEA as the substrates)
packaging
pkg of 10 mg (500 μl)
manufacturer/tradename
Roche
concentration
20 mg/mL (ready-to-use)
optimum pH
8.0(Stability)
9.8(Activity)
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Other Notes
仅用于生命科学研究。不可用于诊断。
Physical form
3 MNaCl (pH 7.6)、5 mM 氯化镁、0.1 mM 氯化锌、30 mM 三乙醇胺溶液。
Preparation Note
活化剂： Mg 2-、Co 2- 和 Mn 2- 离子
General description
重组高活性碱性磷酸酶与天然牛源性碱性磷酸酶具有相同的氨基酸序列。生产工艺中未使用动物来源组分。这消除了动物引起牛海绵状脑病 (BSE) 和其他感染的风险。
重组酶保证了卓越的批间一致性，并完全消除了 BSE 风险。
重组酶保证了卓越的批间一致性，并完全消除了 BSE 风险。
碱性磷酸酶（ALP）是一种水解酶。小牛肠粘膜中的碱性磷酸酶是高活性形式。小牛肠碱性磷酸酶是二聚体糖蛋白，分子量约为 140kDa。
Application
碱性磷酸酶可通过其氨基和碳水化合物基团与其他蛋白质偶联。酶溶液可直接用于偶联，无需预先透析。AP 与 IgG 偶联时，推荐比例为 2.5:1。偶联酶的应用从 ELISA 和微阵列到免疫印迹分析不等。
Biochem/physiol Actions
碱性磷酸酶催化许多磷酸酯的水解，如伯醇酯、仲醇酯、糖苷酯、环醇酯、酚类和胺类。磷酸二酯不发生反应。这种酶水解无机焦磷酸。酶的动力学性质取决于许多因素，如酶纯度、分析中酶的浓度、缓冲液、pH 值等。
碱性磷酸酶（ALP）负责从许多类型的分子（例如核苷酸、蛋白质和生物碱）中除去 5-和 3-位的磷酸基团。它还可以通过催化脂质 A 结构的去磷酸化来修饰脂多糖，从而降低脂多糖的毒性。
Features and Benefits
≥7,000 U/mg（+ 37°C;pNPP；DEA）
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
No data available
flash_point_c
No data available
法规信息
常规特殊物品
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