biological source
bovine intestine
form
suspension
specific activity
~200 units/mg protein (At 25 °C with adenosine as the substrate.)
packaging
pkg of 1 mL (10 mg)
manufacturer/tradename
Roche
technique(s)
activity assay: suitable
color
white
optimum pH
7.0-7.4
solubility
water: miscible
suitability
suitable for chemiluminescence
NCBI accession no.
UniProt accession no.
application(s)
life science and biopharma
foreign activity
AMP-deaminase <0.01%, AP <0.01% (with pre incubation), Guanase <0.01%, NP <0.01%
storage temp.
2-8°C
Quality Level
Gene Information
bovine ... ADA(280712)
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General description
腺苷氨基水解酶。
腺苷脱氨酶（ADA）是一种锌金属酶。
腺苷脱氨酶（ADA）是一种锌金属酶。
Application
化学发光腺苷测定。
将腺苷类似物脱氨基形成相应的肌苷类似物
腺苷脱氨酶（ADA）已与其他试剂一起用于脂解的测定。
腺苷脱氨酶（ADA）已与其他试剂一起用于脂解的测定。
Biochem/physiol Actions
腺苷脱氨酶（ADA）可参与腺苷脱氨基至肌苷，以及2′ 脱氧腺苷脱氨基至2′ -脱氧肌苷的过程。
Physical form
悬浮于3.2M硫酸铵溶液中，pH约为6。存在小颗粒是正常现象。在使用前请搅拌沉淀物再继续使用。
Analysis Note
污染物：<0.01％碱性磷酸酶、AMP-脱氨酶、鸟嘌呤酶和核苷磷酸化酶
Other Notes
仅用于生命科学研究。不可用于诊断。
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
No data available
flash_point_c
No data available
法规信息
动植物源性产品
低风险生物材料
此项目有
Cultured astrocytes do not release adenosine during hypoxic conditions.
Fujita T, et al.
Journal of Cerebral Blood Flow and Metabolism, 32(1), e1-e7 (2012)
Adenosine deaminase from calf intestinal mucosa.
C A ZITTLE
The Journal of biological chemistry, 166(2), 499-503 (1946-12-01)
Mi-Jeong Lee et al.
Methods in enzymology, 538, 49-65 (2014-02-18)
Obesity is reaching epidemic proportions so there is growing interest in the mechanisms that regulates adipose tissue development and function. Although murine adipose cell lines are useful for many mechanistic studies, primary human adipose stromal cells (ASCs), which can be
Alternative substrates for calf intestinal adenosine deaminase. A pre-steady-state kinetic analysis.
D J Porter et al.
The Journal of biological chemistry, 268(4), 2480-2485 (1993-02-05)
The protein fluorescence of adenosine deaminase (ADA) was perturbed during the deamination of adenosine and four analogues of adenosine. The kinetics for the approach to the steady-state during turnover were monitored by fluorescence changes associated with formation of enzymatic intermediates.
Sonia Muñoz-López et al.
Frontiers in endocrinology, 13, 1007801-1007801 (2022-11-22)
Despite great efforts, effective treatment against cancer has not yet been found. However, natural compounds such as the polyphenol resveratrol have emerged as promising preventive agent in cancer therapy. The mode of action of resveratrol is still poorly understood, but
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