biological source
Escherichia coli
form
suspension
mol wt
540 kDa
packaging
pkg of 1,500 U
manufacturer/tradename
Roche
concentration
5.00 mg/mL
technique(s)
activity assay: suitable
optimum pH
7
suitability
suitable for UV spectrophotometry and general use
application(s)
life science and biopharma
shipped in
wet ice
storage temp.
2-8°C
Quality Level
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General description
ββ-半乳糖苷酶或β-D-半乳糖苷半乳糖水解酶是由4条相同的各含1023个氨基酸的肽链组成的四聚体。对D-半乳糖表现高度专一性，完全激活需要K+或Na+和Mg2+。ββ-半乳糖苷酶催化含氧苷键的β-d-吡喃半乳糖苷的反应。
37℃，以2-硝基苯基-β-D-半乳糖苷为底物，25℃，以乳糖为底物，约30 U/mg；用BSA标准化。
Application
ββ-半乳糖苷酶已用于酶联免疫吸附测定(ELISA)。使用本β-半乳糖苷酶可制作酶测定的校准曲线。
Biochem/physiol Actions
切割单糖、寡糖或糖肽的1,4-连接末端半乳糖残基。
Physical form
悬浮于3.2 M硫酸铵溶液中，pH约为6，晶体状
Preparation Note
硫酸铵制剂可以在2至8℃下稳定保存至标签注明的失效日期。可直接用于大多数应用，如乳糖的定量。
在不存在硫酸铵时，应使用Mg2+ (89 mM)和硫醇试剂（1mM β-巯基乙醇，1 mM二硫苏糖醇）稳定β-半乳糖苷酶溶液[Beutler，1984]。硫醇可以减缓由分子内二硫键引起的酶二聚体形成。
活化剂：需要K（50 mM）进行激活（乳糖水解）。
Na（50mM）也是一种活化剂，特别是对于2-硝基苯基-β-D-吡喃半乳糖苷的水解。
在不存在硫酸铵时，应使用Mg2+ (89 mM)和硫醇试剂（1mM β-巯基乙醇，1 mM二硫苏糖醇）稳定β-半乳糖苷酶溶液[Beutler，1984]。硫醇可以减缓由分子内二硫键引起的酶二聚体形成。
活化剂：需要K（50 mM）进行激活（乳糖水解）。
Na（50mM）也是一种活化剂，特别是对于2-硝基苯基-β-D-吡喃半乳糖苷的水解。
Analysis Note
污染物：GIDH、GPT、LDH、MDH和草酰乙酸脱羧酶，每种污染物含量均<0.01％
Other Notes
仅用于生命科学研究。不用于诊断操作。
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
No data available
flash_point_c
No data available
法规信息
常规特殊物品
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