产品名称
过氧化物酶, grade I, from horseradish
optimum pH
6.0-6.5
biological source
horseradish
form
lyophilized (salt-free)
specific activity
≥250 units/mg protein (At 25 °C with guaiacol and H2O2 as the substrates.)
250 U/mg
packaging
pkg of 25,000 U
manufacturer/tradename
Roche
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Analysis Note
污染物：< 0.001% ATP 酶和酸性磷酸酶，< 0.7% 过氧化氢酶
纯度编号：约 3.0 (A403/A275)
纯度编号：约 3.0 (A403/A275)
Application
电子供体的氧化（例如，双键的环氧化）。过氧化物酶可通过其氨基以及其碳水化合物部分与其他蛋白质偶联。复溶溶液可直接用于偶联，无需预先透析。已用于配体印迹。
过氧化物酶 (POD) 已被用于 AUR（Amplex 红外线测定）中测定由分解的段塞细胞产生的胞外 H2O2。
Biochem/physiol Actions
过氧化物酶 (POD) 参与大量有机化合物的脱氢，包括酚类和芳香胺、对苯二酚和对苯二酚胺，主要是联苯胺衍生物。
General description
供体：过氧化氢氧化还原酶
过氧化物酶 (POD) (E.C. 1.11.1.7) 在大多数水果和蔬菜中广泛表达。
Other Notes
仅用于生命科学研究。不可用于诊断。
Preparation Note
10 mg/mL 水溶液：澄清，红棕色；pH 值：6.0 至 7.0。
催化剂：氨、吡啶、咪唑，均在 pH7 以上；洗涤剂。
工作溶液：PBS（磷酸盐缓冲盐水）或水（起始浓度 20 mg/mL）。
工作溶液：PBS（磷酸盐缓冲盐水）或水（起始浓度 20 mg/mL）。
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
动植物源性产品
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