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Merck
CN

10165948001

Roche

RNA, MS2

from bacteriophage MS2

别名:

RNA, 核糖核酸

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关于此项目

UNSPSC Code:
41106311
NACRES:
NA.54
Biological source:
bacteriophage MS2
Form:
solution
Solubility:
water: miscible
Storage temp.:
−20°C (−15°C to −25°C)
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biological source

bacteriophage MS2

form

solution

mol wt

~1200 kDa

packaging

pkg of 500 μL (10 A260 units)

manufacturer/tradename

Roche

technique(s)

RNA extraction: suitable

color

colorless

solubility

water: miscible

storage temp.

−20°C (−15°C to −25°C)

General description

MS2噬菌体RNA由3569个核苷酸组成。MS2噬菌体在分子生物学研究中是模式系统,可用于病毒RNA复制,感染细胞翻译和生理学研究。MS2 RNA编码三个病毒多肽链,即蛋白质A、外壳蛋白和RNA复制酶复合物。蛋白质A和外壳蛋白组成了MS2病毒的结构。

Application

RNA,MS2用于:
  • 评估RNA定量方法的标准品
  • RNA提取
  • 单管荧光PERT检测(STF-PERT),对不同逆转录病毒类型进行准确定量
  • 各种研究,在体内体外使用天然RNA的蛋白合成系统,起始、延长或终止多肽链合成。
  • 结构和功能研究。
  • 在cDNA合成过程中稳定RNA
  • 在RNA提取或分离过程中提高RNA产量

Preparation Note

工作溶液:Storage buffer: 10 mM Tris-HCl,1 mM EDTA,pH 7.0。

Analysis Note

典型分析:根据现在的质量控制步骤,不会有蛋白质和宿主核酸。

Other Notes

仅用于生命科学研究。不可用于诊断。
链长度3569个核苷酸

存储类别

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

No data available

flash_point_c

No data available

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

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Dennis M Timmerman et al.
Cancers, 13(17) (2021-09-11)
MicroRNAs (miRNAs) are short, non-coding RNAs involved in translation regulation. Dysregulation has been identified in cancer cells. miRNAs can be secreted and detectable in body fluids; therefore, they are potential liquid-biopsy biomarkers. The miR-371a-3 cluster members are an example, monitoring
Kirstine Kirkegaard et al.
Journal of assisted reproduction and genetics, 37(9), 2199-2209 (2020-07-19)
miRNAs have been suggested as biomarkers of embryo viability; however, findings from preliminary studies are divergent. Furthermore, the presence of other types of small RNA molecules remains to be investigated. The purpose of this study was to perform a comprehensive
Yun Kun Ma et al.
Journal of virological methods, 157(2), 133-140 (2009-02-03)
PCR-based reverse transcriptase (RT) assays are highly sensitive for broad detection of retroviruses. These assays are currently used for demonstrating the absence of retroviral contamination in vaccines and can also be applied to clinical and laboratory research to investigate low-virus
Michael Eikmans et al.
International journal of molecular sciences, 21(11) (2020-06-12)
About 10-15% of couples who want to conceive suffer from subfertility, while in 30% of these cases, a male factor plays a role. Levels of particular microRNAs in seminal plasma, including those involved in spermatogenesis, may serve as an indicative
Rom S Leidner et al.
PloS one, 8(3), e57841-e57841 (2013-03-09)
Genome-wide platforms for high-throughput profiling of circulating miRNA (oligoarray or miR-Seq) offer enormous promise for agnostic discovery of circulating miRNA biomarkers as a pathway for development in breast cancer detection. By harmonizing data from 15 previous reports, we found widespread

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