biological source
bacteriophage MS2
form
solution
mol wt
~1200 kDa
packaging
pkg of 500 μL (10 A260 units)
manufacturer/tradename
Roche
technique(s)
RNA extraction: suitable
color
colorless
solubility
water: miscible
storage temp.
−20°C (−15°C to −25°C)
General description
MS2噬菌体RNA由3569个核苷酸组成。MS2噬菌体在分子生物学研究中是模式系统,可用于病毒RNA复制,感染细胞翻译和生理学研究。MS2 RNA编码三个病毒多肽链,即蛋白质A、外壳蛋白和RNA复制酶复合物。蛋白质A和外壳蛋白组成了MS2病毒的结构。
Application
RNA,MS2用于:
- 评估RNA定量方法的标准品
- RNA提取
- 单管荧光PERT检测(STF-PERT),对不同逆转录病毒类型进行准确定量
- 各种研究,在体内或体外使用天然RNA的蛋白合成系统,起始、延长或终止多肽链合成。
- 结构和功能研究。
- 在cDNA合成过程中稳定RNA
- 在RNA提取或分离过程中提高RNA产量
Preparation Note
工作溶液:Storage buffer: 10 mM Tris-HCl,1 mM EDTA,pH 7.0。
Analysis Note
典型分析:根据现在的质量控制步骤,不会有蛋白质和宿主核酸。
Other Notes
仅用于生命科学研究。不可用于诊断。
链长度3569个核苷酸
存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
No data available
flash_point_c
No data available
法规信息
常规特殊物品
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