biological source
bovine liver
form
lyophilized
specific activity
10 U/mg, ~10 units/mg protein (at 25 °C with 2-oxoglutarate as the substrate, and ADP as the activator.)
packaging
pkg of 3,000 U
manufacturer/tradename
Roche
concentration
≥10-20 % (w/w)
technique(s)
activity assay: suitable
color
white
optimum pH
8
solubility
water: 20 mg/mL, colorless
suitability
suitable for UV spectrophotometry and general use
UniProt accession no.
application(s)
life science and biopharma
foreign activity
ADH <0.00500%, LDH <0.00500%, MDH <0.00500%
shipped in
wet ice
storage temp.
2-8°C
Gene Information
bovine ... GLUD1(281785)
General description
GDH（谷氨酸脱氢酶）是含有449个残基的六聚体，其位于线粒体中。它可充当氨基酸氧化和尿素生成之间的分支酶。L-谷氨酸：NAD（P）+氧化还原酶参与脱氨。
在+25°C下，以2-氧代戊二酸为底物、ADP为激活剂时，冻干物酶活性约为10 U/mg（120 U/mg酶蛋白）。
Application
在葡萄酒酵母发酵过程中，谷氨酸脱氢酶用于通过酶分析测定残留铵。它作为NAD（P）和NAD（P）H 辅助因子循环系统的组分。
Analysis Note
污染物：分别含有<0.005％的ADH、LDH和MDH
Other Notes
GIDH（310,000至350,000）的最小活性结构是一个55,400D的六聚体亚基。该六聚体能够可逆地自组装形成具有多达八个六聚体(2,200 kD)的聚合物。
仅用于生命科学研究。不可用于诊断。
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
低风险生物材料
动植物源性产品
此项目有
Disruption of the cell wall integrity gene ECM33 results in improved fermentation by wine yeast
Jin Zhang
Metabolic engineering (2018)
T J Stillman et al.
Journal of molecular biology, 234(4), 1131-1139 (1993-12-20)
We have solved the structure of the binary complex of the glutamate dehydrogenase from Clostridium symbiosum with glutamate to 1.9 A resolution. In this complex, the glutamate side-chain lies in a pocket on the enzyme surface and a key determinant
G Hughes et al.
Analytica chimica acta, 933, 82-88 (2016-08-09)
A reagentless glutamate biosensor was applied to the determination of glutamate released from liver hepatocellular carcinoma cells (HepG2) in response to toxic challenge from various concentrations of paracetamol. A screen printed carbon electrode (SPCE) containing the electrocatalyst Meldola's Blue (MB-SPCE)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 10197734001 | 04061838668707 |