产品名称
GTP-γ-S, Tetralithium salt
color
white
description
C10H12N5O13P3SLi4
assay
87% (HPLC)
form
solid
mol wt
Mr 539.2 (GTP-γ-S)
Mr 563.0 (GTP-γ-S-Li4)
composition
GDP, ≤12% HPLC
GMP, ≤1% HPLC
GTP, ≤2% HPLC
packaging
pkg of 10 mg
manufacturer/tradename
Roche
storage condition
protect from light
concentration
97%
technique(s)
activity assay: suitable
solubility
water: soluble
storage temp.
−20°C (−15°C to −25°C)
Quality Level
Analysis Note
吸光度: 在水溶液中采用254nm吸光法测定含量。
条件：
条件：
- 约10 mg/ml双蒸水。
- 稀释度：9.8 ml双蒸水 + 0.2 ml样品。
Application
GTP-γ-S已用于：
- 在G蛋白激活检测中评估蛋白酶激活受体4(PAR4)的功能
- 用于G蛋白α亚基(Gα)的荧光法鸟嘌呤核苷酸交换测定
- 监测磷脂酶Cγ2突变体的Rac家族小鸟苷三磷酸酶（GTP酶）2 (Rac2)刺激活性
Biochem/physiol Actions
鸟苷5′-O-(3-硫代三磷酸)(GTP-γ-S)可激活鸟嘌呤-核苷酸结合蛋白，可通过酶法缓慢水解。它对鸟苷三磷酸酶（GTP酶）的抑制作用强于鸟苷三磷酸(GTP)。
General description
鸟苷5′-O-(3-硫代三磷酸)四锂盐(GTP-γ-S)是GTP的不可水解类似物。它是光感受器中磷酸二酯酶的抑制剂。
Other Notes
仅用于生命科学研究。不可用于诊断。
Preparation Note
-15至-25°C环境下干燥储存粉末；pH值约为7。应在-15至-25°C下可稳定保存六个月。
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Gilbert Di Paolo, Markus R. Wenk
Lipids, 3-18 (2012)
Stephen R Sprang
Biopolymers, 105(8), 449-462 (2016-03-22)
This review addresses the regulatory consequences of the binding of GTP to the alpha subunits (Gα) of heterotrimeric G proteins, the reaction mechanism of GTP hydrolysis catalyzed by Gα and the means by which GTPase activating proteins (GAPs) stimulate the
S Nawy et al.
Neuron, 7(4), 677-683 (1991-10-01)
Transmitter release from photoreceptors is decreased by light, resulting in a conductance increase in depolarizing bipolar cells. Addition of exogenous cGMP through a patch pipette to depolarizing bipolar cells from slices of dark-adapted tiger salamander retina resulted in an enhancement
K Sasaki et al.
Nature, 325(6101), 259-262 (1987-01-15)
Recently, a GTP-binding protein sensitive to islet activating protein (IAP) has been suggested to be important in producing K+-currents when the muscarinic receptor of the atrial muscle is activated by acetylcholine (ACh). Here we confirm the blocking effects of IAP
Baisen Zeng et al.
Structure (London, England : 1993), 27(7), 1137-1147 (2019-06-04)
Ric-8A is a 530-amino acid cytoplasmic molecular chaperone and guanine nucleotide exchange factor (GEF) for i, q, and 12/13 classes of heterortrimeric G protein alpha subunits (Gα). We report the 2.2-Å crystal structure of the Ric-8A Gα-binding domain with GEF
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