产品名称
柠檬酸裂解酶(CL), from Klebsiella pneumoniae (Aerobacter aerogenes)
biological source
bacterial (Klebsiella pneumoniae)
form
lyophilized
specific activity
≥0.25 U/mg (At 25 °C with citrate as the substrate.)
packaging
pkg of 120 U
manufacturer/tradename
Roche
technique(s)
activity assay: suitable
color
light cream
optimum pH
8.0-9.0
solubility
water: soluble
NCBI accession no.
UniProt accession no.
application(s)
life science and biopharma
foreign activity
ICDH <0.05% (NAD as coenzyme)
NADH oxidase <0.05%
storage temp.
2-8°C
Quality Level
Application
它适用于采用微量滴定板测量柠檬酸盐浓度的酶学测定。
Analysis Note
污染物：ICDH(NAD依赖型)和“NADH氧化酶”，两者均<0.05%
General description
柠檬酸裂解酶被认为是底物诱导酶，有助于催化柠檬酸盐发生可逆的醛醇裂解，形成草乙酸和乙酸盐。该酶适用于柠檬酸盐的缺氧异化。
Other Notes
仅用于生命科学研究。不可用于诊断。
Physical form
冻干品，通过BSA、蔗糖、MgSO4和EDTA增强稳定性。注：以双蒸水为溶剂的溶液pH值约为7.0。酶可以再次冻干。
Preparation Note
激活剂：Mg2+ (Km = 3.0 mM，pH 7.4，25 °C)；其他二价金属离子(Mn2+，Fe2+，Zn2+)
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
含少量动物源组分生物产品
常规特殊物品
此项目有
Agata M Pudlik et al.
Journal of bacteriology, 193(3), 706-714 (2010-12-01)
Carbohydrate/citrate cometabolism in Lactococcus lactis results in the formation of the flavor compound acetoin. Resting cells of strain IL1403(pFL3) rapidly consumed citrate while producing acetoin when substoichiometric concentrations of glucose or l-lactate were present. A proton motive force was generated
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