产品名称
DNA,λ, from bacteriophage lambda cl857 Sam 7
form
solution
mol wt
32,300 kDa
packaging
pkg of 1 mL
manufacturer/tradename
Roche
storage condition
avoid repeated freeze/thaw cycles
concentration
250 μg/mL
impurities
RNA, none detected
nuclease, none detected
color
colorless
solubility
water: miscible
shipped in
dry ice
storage temp.
−20°C (−15°C to −25°C)
Quality Level
Analysis Note
限制性核酸内切酶 EcoR I 和 Hind III 酶切后的 DNA 在凝胶电泳中显示野生型 λDNA 的典型酶切图谱。
Application
λDNA(DNA, lambda)已用于在基因组DNA分离前注入外周血白细胞(PBL)。它也用于制备生物素化双链DNA(dsDNA)底物。
General description
噬菌体 λ 的 DNA 含有 48,502 个核苷酸对，形成线性双链。这种 DNA 分子的 5′ 端有 12 个核苷酸的自互补单链突起，可退火形成圆圈。进入宿主细胞后，DNA 在 DNA 连接酶存在下环化。许多这样的圆圈是在溶菌感染早期产生的。在复制的后期，这些单独的环/环被称为滚环复制的过程转化成线性连通体。线性连体在包装过程中被酶 DNA 终止酶切割形成单体染色体单位。
Other Notes
仅用于生命科学研究。不可用于诊断。
链长 48,502 bp
Physical form
溶液，250μg/ml，10 mM Tris-HCl，1 mM EDTA，pH 8.0，即用型
Preparation Note
使用前储存于-15至-25°C。解冻后，储存于2-8°C。
存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
No data available
flash_point_c
No data available
法规信息
常规特殊物品
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