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检测方案
>99% (titration)
形式
solid
包装
pkg of 500 g
制造商/商品名称
Roche
技术
FISH: suitable
pH值(酸碱度)
7.0-9.0
有效pH范围
7.0-9.0
pKa (25 °C)
8.1
吸光度
<0.02 at 300 nm at 100 mg/mL
储存温度
room temp
SMILES字符串
Cl.NC(CO)(CO)CO
InChI
1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
InChI key
QKNYBSVHEMOAJP-UHFFFAOYSA-N
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应用
孵育混合物中的缓冲试剂。它也被用作裂解缓冲液及TE(Tris-EDTA)缓冲液的一种组分。
特点和优势
晶体
质量
纯度:>99% Tris-HCl(滴定测量)
重金属:<1 ppm Pb及Fe
重金属:<1 ppm Pb及Fe
其他说明
所有缓冲液的pH值都与温度和浓度有关。对于 Tris 缓冲液,温度每降低 1°C,pH 就会增加约 0.03 单位,每稀释 10 倍,pH 会降低 0.03-0.05 单位。对于一些精准的应用,请使用经过仔细校准的pH计和玻璃/甘汞组合电极。
法律信息
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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MNase Digestion for Nucleosome Mapping in Neurospora.
Bio-protocol null
STAR protocols, 1(3), 100135-100135 (2020-12-31)
Protein lysine methylation mediates a variety of biological processes, and their dysregulation has been established to play pivotal roles in human disease. A number of these sites constitute attractive drug targets. However, systematic identification of methylation sites is challenging and
STAR protocols, 1(3), 100162-100162 (2020-12-31)
Secretome analysis is crucial to unravel extracellular processes. However, secreted proteins are difficult to detect in mass-spectrometry-based analysis due to contamination of serum proteins deriving from cell culture media and to high glycosylation, which hampers tryptic digestion. Secret3D workflow is
STAR protocols, 2(3), 100652-100652 (2021-07-27)
16S rRNA gene sequencing enables microbial community profiling, but recovering fecal DNA from extremely premature infants is challenging. Here, we describe an optimized protocol for fecal DNA isolation, library preparation for 16S rRNA gene sequencing, taxonomy assignation, and statistical analyses.
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