Merck
CN

10838039001

Roche

纤连蛋白

from human plasma

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别名:
纤连蛋白 溶液 来源于人类成纤维细胞
MDL编号:

生物来源

human plasma

质量水平

无菌性

non-sterile; 0.2 μm filtered

检测方案

95% (SDS-PAGE)

形式

lyophilized (clear, colorless solution after reconstitution)

分子量

440 kDa

包装

pkg of 5 mg

manufacturer/tradename

Roche

储存条件

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

UniProt登记号

储存温度

2-8°C

Gene Information

human ... FN1(2335)

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一般描述

纤连蛋白负责细胞与细胞外基质的相互作用。参与细胞的黏附、迁移、生长和分化。是血浆和其他体液的可溶性成分。此外,它也是不溶性细胞外基质的一部分。

特异性

对大多数哺乳动物细胞有活性

应用

纤维连接蛋白促进许多细胞的附着和随后的扩散。用于培养皿的包被。

测试方法

促进 3T3 (NR6) 细胞粘附的检测(XTT 切割)

外形

在冻干过滤通过 0.2μm 孔径的膜之前,冷冻干燥物(每 mg 纤连蛋白含有 1.126mg 甘氨酸和 0.058mg 氯化钠)。

制备说明

工作浓度: 5μg/cm 2
5μg/cm 2 对于细胞培养容器的包衣
储存条件(工作液):-15°C 至-25°C
复溶溶液在-15°C 至-25°C 下稳定。
注意: 将复溶溶液分等份储存于-15 至-25°C 条件下。
避免反复冻融。

重悬

分别加入 1 或 5 mL 无菌水,得到最终浓度为 1 mg/mL 的溶液。在 37°C 下孵育 30 至 60 min 以溶解。 不要搅拌。
复溶后,溶液可能含有少量不溶性聚集物。这些聚集体是纤连蛋白固有的现象,不影响产品性能。

其他说明

仅用于生命科学研究。不可用于诊断。

免责声明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

does not flash

闪点(°C)

does not flash

法规信息

常规特殊物品

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