biological source
bovine kidney
form
lyophilized
specific activity
~20 mU/mL
packaging
pkg of 5 × 3 mL (5 x 400 μg)
manufacturer/tradename
Roche
technique(s)
activity assay: suitable
color
white to light yellow
solubility
water: soluble
application(s)
life science and biopharma
storage temp.
20-25°C
General description
Standard for the determination of NAG activity in urine research samples with the aid of the Test-Combination N-acetyl-β-D-glucosaminidase.
N-Acetyl-β-D-Glucosaminidase is a high molecular weight lysosomal enzyme existing as two isofomrs, Isoenzyme-A and Isoenzyme-B. It is present in human kidney. It has high molecular weight. It is highly active in renal proximal tubular cells. It has been reported that overexpressed urine-NAG reflects the proximal tubular dysfunction of the kidney.
N-Acetyl-β-D-Glucosaminidase is a high molecular weight lysosomal enzyme existing as two isofomrs, Isoenzyme-A and Isoenzyme-B. It is present in human kidney. It has high molecular weight. It is highly active in renal proximal tubular cells. It has been reported that overexpressed urine-NAG reflects the proximal tubular dysfunction of the kidney.
Application
The NAG standard is used for the determination of NAG activity in urine in life science research applications, with the aid of N-acetyl-β-D-glucosaminidase. It can be used in automatic analyzers.
Preparation Note
Storage conditions (working solution): 2 to 8 °C
Stability of the standard solution
The solution is stable for at least 1 month stored at 2 to 8 °C, protected from light.
Stability of the standard solution
The solution is stable for at least 1 month stored at 2 to 8 °C, protected from light.
Preparation of the standard solution
Dissolve contents of one bottle with 3 ml double distilled water, close bottle and wait for 5 minutes. Dissolve contents completely by gentle swirling. Avoid creating foam.
Dissolve contents of one bottle with 3 ml double distilled water, close bottle and wait for 5 minutes. Dissolve contents completely by gentle swirling. Avoid creating foam.
Keep protected from light.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Volume Activity: The activity of the NAG standard is approximately 20 mU N-acetyl-β-D-glucosaminidase from beef kidney per ml. For exact values, see data printed on the label.
hcodes
pcodes
Hazard Classifications
Aquatic Chronic 3
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
低风险生物材料
动植物源性产品
此项目有
Ravi Babu Kalahasthi et al.
Journal of occupational medicine and toxicology (London, England), 2, 5-5 (2007-07-31)
The present study was carried out to determine the effect of cadmium exposure on Urinary N-acetyl-beta -D-glucosaminidase and its isoenzymes A and B in workers exposed at cadmium plating. 50 subjects using cadmium during cadmium plating formed the study group.
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