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Merck
CN

11051482001

Roche

胸腺嘧啶核苷5′-三磷酸 钠盐

Lithium salt

别名:

TTP, dTTP

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关于此项目

经验公式(希尔记法):
C10H16N2O14P3Na
化学文摘社编号:
分子量:
504.15
UNSPSC Code:
41116100
PubChem Substance ID:
MDL number:
NACRES:
NA.54
Assay:
≥98%
Form:
solution
Storage temp.:
−20°C
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assay

≥98%

form

solution

usage

sufficient for 6,250 standard reactions (PCR)

packaging

pkg of 250 μL (25 μmol)

manufacturer/tradename

Roche

concentration

100 mM

technique(s)

DNA amplification: suitable, DNA sequencing: suitable

storage temp.

−20°C

SMILES string

[NaH].[P](=O)(O[P](=O)(OCC1OC(CC1O)N2C=C(C(=O)NC2=O)C)O)(O[P](=O)(O)O)O

InChI

1S/C10H17N2O14P3.Na.H/c1-5-3-12(10(15)11-9(5)14)8-2-6(13)7(24-8)4-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h3,6-8,13H,2,4H2,1H3,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;

InChI key

KAELIXTVSANDSE-UHFFFAOYSA-N

General description

dTTP is a 100 mM clear, colorless solution of lithium salt (pH 7).
dTTP solution in water (pH 7), 100 mM, lithium salt.

Application

Use dTTP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.
It has been used in PCR fingrprinting and PCR techniques.

Analysis Note

Typical analysis:
  • ≥98% dTTP (HPLC), ≤1.5% dTDP (HPLC), ≤0.5% dTMP (HPLC)
  • Absorbance determination A250/A260, A280/A260, A290/A260
  • DNase activity negative, according to the current Quality Control procedures
  • Suitability for PCR

Other Notes

1 μmol dTTP = 9.5 A260units
For life science research only. Not for use in diagnostic procedures.

Our PCR Grade deoxynucleotides are even purer than the lithium salts, to ensure excellent performance in your PCRs. PCR Grade deoxynucleotides contain the sodium salt of the nucleotide in PCR Grade Water.
For your convenience, some of our deoxynucleotides are also available in:
  • nucleotide sets (all 4 nucleotides in separate vials)
  • ready-to-use nucleotide mixes (premixed solutions of all 4 nucleotides)

They are also vital components of:
  • kits (dNTPs, buffer, and polymerase in separate vials)
  • ready-to-use mixes [polymerase, nucleotides, and buffer (premixed 2x solutions)]


存储类别

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

No data available

flash_point_c

No data available



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1105148200104061838697530