Merck
CN
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文件

11051482001

Roche

胸腺嘧啶核苷5′-三磷酸 钠盐

Lithium salt

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别名:
TTP, dTTP
经验公式（希尔记法）:
C10H16N2O14P3Na
CAS号:
18423-43-3
分子量:
504.15
MDL编号:
MFCD00080338
PubChem化学物质编号:
329749302

质量水平

100

检测方案

≥98%

形式

solution

用途

sufficient for 6,250 standard reactions (PCR)

包装

pkg of 250 μL (25 μmol)

manufacturer/tradename

Roche

浓度

100 mM

technique(s)

DNA amplification: suitable
DNA sequencing: suitable

储存温度

−20°C

InChI

1S/C10H17N2O14P3.Na.H/c1-5-3-12(10(15)11-9(5)14)8-2-6(13)7(24-8)4-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h3,6-8,13H,2,4H2,1H3,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;

InChI key

KAELIXTVSANDSE-UHFFFAOYSA-N

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相关类别

一般描述

dTTP is a 100 mM clear, colorless solution of lithium salt (pH 7).
dTTP solution in water (pH 7), 100 mM, lithium salt.

应用

Use dTTP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.
It has been used in PCR fingrprinting and PCR techniques.

质量

Typical analysis:
  • ≥98% dTTP (HPLC), ≤1.5% dTDP (HPLC), ≤0.5% dTMP (HPLC)
  • Absorbance determination A250/A260, A280/A260, A290/A260
  • DNase activity negative, according to the current Quality Control procedures
  • Suitability for PCR

单位定义

1 μmol dTTP = 9.5 A260units

其他说明

For life science research only. Not for use in diagnostic procedures.

Our PCR Grade deoxynucleotides are even purer than the lithium salts, to ensure excellent performance in your PCRs. PCR Grade deoxynucleotides contain the sodium salt of the nucleotide in PCR Grade Water.
For your convenience, some of our deoxynucleotides are also available in:
  • nucleotide sets (all 4 nucleotides in separate vials)
  • ready-to-use nucleotide mixes (premixed solutions of all 4 nucleotides)

They are also vital components of:
  • kits (dNTPs, buffer, and polymerase in separate vials)
  • ready-to-use mixes [polymerase, nucleotides, and buffer (premixed 2x solutions)]

储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

No data available

闪点(°C)

No data available

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Wieland Meyer et al.
Emerging infectious diseases, 9(2), 189-195 (2003-02-27)
A network was established to acquire basic knowledge of Cryptococcus neoformans in IberoAmerican countries. To this effect, 340 clinical, veterinary, and environmental isolates from Argentina, Brazil, Chile, Colombia, Mexico, Peru, Venezuela, Guatemala, and Spain were typed by using M13 polymerase
Luciana Trilles et al.
Memorias do Instituto Oswaldo Cruz, 103(5), 455-462 (2008-09-18)
The molecular types of 443 Brazilian isolates of Cryptococcus neoformans and Cryptococcus gattii were analyzed to determine their geographic distribution within Brazil and their underlying host conditions. The following data, imported from previous epidemiological studies as well as two culture
A Pillay et al.
Journal of clinical microbiology, 42(8), 3853-3856 (2004-08-07)
An immunochromatographic strip test, Xenostrip-Tv, was compared to wet mount and PCR for the diagnosis of Trichomonas vaginalis infection in women. Of 428 specimens tested, 54 (12.6%) were positive by an "expanded gold standard," defined as either a positive wet

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