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Merck
CN

11089161001

Roche

链霉抗生物素蛋白-AP缀合物

solution, pkg of 1 mL (1,000U)

别名:

链霉亲和素

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UNSPSC Code:
12352200
NACRES:
NA.54
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产品名称

链霉抗生物素蛋白-AP缀合物, solution, pkg of 1 mL (1,000U)

form

solution

packaging

pkg of 1 mL (1,000U)

manufacturer/tradename

Roche

storage temp.

2-8°C

Quality Level

Application

链霉亲和素-AP 已用于检测生物素化化合物,用于:
  • ELISA
  • 免疫组织化学
  • 蛋白印迹

General description

链霉亲和素与碱性磷酸酶 (AP) 共价偶联,用于制备链霉亲和素-AP 偶联物。

碱性磷酸酶
碱性磷酸酶常被用作酶免疫测定的标记物。AP 将敏感底物 X-磷酸盐/NBT 转化为稳定的水溶性产物。

Other Notes

仅用于生命科学研究。不可用于诊断。

Physical form

溶液,在 50 mM 三乙醇胺缓冲液、3 MNaCl、1 mM MgCl 2 、0.1 mM ZnCl 2 、10 mg/mL 牛血清白蛋白 (pH 7.6) 中稳定

Preparation Note

工作溶液: 50-1,000 mU/ml(即贮备液可稀释)。将缀合物储备溶液在 100 mM Tris-HCl、150 mM NaCl (pH 7.5) 中稀释。

工作浓度: 缀合物的工作浓度取决于应用和底物。标示浓度应作为指导。

ELISA:50-200 mU/ml(1:5000-1:20,000 稀释)
免疫组织化学/细胞化学:250-1,000 mU/ml(1:1000-1:4000 稀释)
免疫印迹:200-500 mU/ml(1:2000-1:5000 稀释)

稳定性: 浓缩储备液在 2 ~ 8°C 下可稳定储存至标签上打印的有效期为止。 切勿冻结!

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

No data available

flash_point_c

No data available

法规信息

常规特殊物品
含少量动物源组分生物产品
此项目有

历史批次信息供参考:

分析证书(COA)

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Manuela Sauter et al.
STAR protocols, 3(3), 101664-101664 (2022-09-14)
Different types of immune cells are involved in atherogenesis and may act atheroprotective or atheroprogressive. Here, we describe an in vitro approach to analyze CD11c+ cells and CD11c+-derived ApoE in atherosclerosis. The major steps include harvesting mouse bone marrow, plating
Muhammad Assad Aslam et al.
EMBO reports, 22(2), e51184-e51184 (2021-01-08)
Differentiation of naïve peripheral B cells into terminally differentiated plasma cells is characterized by epigenetic alterations, yet the epigenetic mechanisms that control B-cell fate remain unclear. Here, we identified a role for the histone H3K79 methyltransferase DOT1L in controlling B-cell
Ablation of BMP signaling hampers the blastema formation in Poecilia latipinna by dysregulating the extracellular matrix remodeling and cell cycle turnover
Patel S, et al.
Zoology (Jena, Germany), 133, 17-26 (2019)
M Degerman Gunnarsson et al.
Dementia and geriatric cognitive disorders, 29(3), 204-212 (2010-03-25)
The positron emission tomography (PET) radiotracer Pittsburgh Compound-B (PIB) is an in vivo ligand for measuring beta-amyloid (Abeta) load. Associations between PET PIB and cerebrospinal fluid (CSF) Abeta1-42 and apolipoprotein E epsilon4 (APOE epsilon4) have been observed in several studies
Manuela Sauter et al.
iScience, 25(1), 103677-103677 (2022-01-18)
Atherosclerosis is studied in models with dysfunctional lipid homeostasis-predominantly the ApoE-/- mouse. The role of antigen-presenting cells (APCs) for lipid homeostasis is not clear. Using a LacZ reporter mouse, we showed that CD11c+ cells were enriched in aortae of ApoE-/-

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