form
solution
packaging
pkg of 1 mL (1,000U)
manufacturer/tradename
Roche
storage temp.
2-8°C
General description
链霉亲和素与碱性磷酸酶 (AP) 共价偶联,用于制备链霉亲和素-AP 偶联物。
碱性磷酸酶
碱性磷酸酶常被用作酶免疫测定的标记物。AP 将敏感底物 X-磷酸盐/NBT 转化为稳定的水溶性产物。
碱性磷酸酶
碱性磷酸酶常被用作酶免疫测定的标记物。AP 将敏感底物 X-磷酸盐/NBT 转化为稳定的水溶性产物。
Application
链霉亲和素-AP 已用于检测生物素化化合物,用于:
- ELISA
- 免疫组织化学
- 蛋白印迹
Physical form
溶液,在 50 mM 三乙醇胺缓冲液、3 MNaCl、1 mM MgCl 2 、0.1 mM ZnCl 2 、10 mg/mL 牛血清白蛋白 (pH 7.6) 中稳定 。
Preparation Note
工作溶液: 50-1,000 mU/ml(即贮备液可稀释)。将缀合物储备溶液在 100 mM Tris-HCl、150 mM NaCl (pH 7.5) 中稀释。
工作浓度: 缀合物的工作浓度取决于应用和底物。标示浓度应作为指导。
ELISA:50-200 mU/ml(1:5000-1:20,000 稀释)
免疫组织化学/细胞化学:250-1,000 mU/ml(1:1000-1:4000 稀释)
免疫印迹:200-500 mU/ml(1:2000-1:5000 稀释)
稳定性: 浓缩储备液在 2 ~ 8°C 下可稳定储存至标签上打印的有效期为止。 切勿冻结!
工作浓度: 缀合物的工作浓度取决于应用和底物。标示浓度应作为指导。
ELISA:50-200 mU/ml(1:5000-1:20,000 稀释)
免疫组织化学/细胞化学:250-1,000 mU/ml(1:1000-1:4000 稀释)
免疫印迹:200-500 mU/ml(1:2000-1:5000 稀释)
稳定性: 浓缩储备液在 2 ~ 8°C 下可稳定储存至标签上打印的有效期为止。 切勿冻结!
Other Notes
仅用于生命科学研究。不可用于诊断。
Still not finding the right product?
Explore all of our products under 链霉抗生物素蛋白-AP缀合物
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
No data available
flash_point_c
No data available
法规信息
常规特殊物品
低风险生物材料
此项目有
Ablation of BMP signaling hampers the blastema formation in Poecilia latipinna by dysregulating the extracellular matrix remodeling and cell cycle turnover
Patel S, et al.
Zoology (Jena, Germany), 133, 17-26 (2019)
Manuela Sauter et al.
STAR protocols, 3(3), 101664-101664 (2022-09-14)
Different types of immune cells are involved in atherogenesis and may act atheroprotective or atheroprogressive. Here, we describe an in vitro approach to analyze CD11c+ cells and CD11c+-derived ApoE in atherosclerosis. The major steps include harvesting mouse bone marrow, plating
Johan L K Van Hove et al.
Molecular genetics and metabolism, 95(4), 201-205 (2008-11-01)
We investigated in a patient with holocarboxylase synthetase deficiency, the relation between the biochemical and genetic factors of the mutant protein with the pharmacokinetic factors of successful biotin treatment. A girl exhibited abnormal skin at birth, and developed in the
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 11089161001 | 04061838250650 |
