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Merck
CN

11089161001

Roche

链霉抗生物素蛋白-AP缀合物

solution, pkg of 1 mL (1,000U)

别名:

链霉亲和素

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UNSPSC Code:
12352200
NACRES:
NA.54
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form

solution

packaging

pkg of 1 mL (1,000U)

manufacturer/tradename

Roche

storage temp.

2-8°C

Quality Level

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General description

链霉亲和素与碱性磷酸酶 (AP) 共价偶联,用于制备链霉亲和素-AP 偶联物。

碱性磷酸酶
碱性磷酸酶常被用作酶免疫测定的标记物。AP 将敏感底物 X-磷酸盐/NBT 转化为稳定的水溶性产物。

Application

链霉亲和素-AP 已用于检测生物素化化合物,用于:
  • ELISA
  • 免疫组织化学
  • 蛋白印迹

Physical form

溶液,在 50 mM 三乙醇胺缓冲液、3 MNaCl、1 mM MgCl 2 、0.1 mM ZnCl 2 、10 mg/mL 牛血清白蛋白 (pH 7.6) 中稳定

Preparation Note

工作溶液: 50-1,000 mU/ml(即贮备液可稀释)。将缀合物储备溶液在 100 mM Tris-HCl、150 mM NaCl (pH 7.5) 中稀释。

工作浓度: 缀合物的工作浓度取决于应用和底物。标示浓度应作为指导。

ELISA:50-200 mU/ml(1:5000-1:20,000 稀释)
免疫组织化学/细胞化学:250-1,000 mU/ml(1:1000-1:4000 稀释)
免疫印迹:200-500 mU/ml(1:2000-1:5000 稀释)

稳定性: 浓缩储备液在 2 ~ 8°C 下可稳定储存至标签上打印的有效期为止。 切勿冻结!

Other Notes

仅用于生命科学研究。不可用于诊断。

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

No data available

flash_point_c

No data available

法规信息

常规特殊物品
低风险生物材料
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历史批次信息供参考:

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