产品名称
三磷酸鸟苷, =98% (HPLC), solution, pkg of 400 μL (100 mM; 40 μmol)
description
Lithium salt
assay
98% (HPLC)
form
solution
packaging
pkg of 400 μL (100 mM; 40 μmol)
manufacturer/tradename
Roche
storage temp.
−20°C
Analysis Note
典型分析：
- ≥8%GTP (HPLC)，≤1.5%GDP (HPLC)，≤0.5%GMP (HPLC)。
- 根据当前质量控制程序检测是否存在 rna 酶。
Application
GTP 被用于 COPII 包被的前胶原 I 载体的无细胞生成 和标记转录延伸复合物。
GTP，锂盐，适用于体外 RNA 转录等应用。
General description
GTP 为 100 mM 锂盐 (pH 7) 溶液。
鸟苷三磷酸 (GTP) 是一种嘌呤核苷三磷酸。该核苷酸在转录过程中主要作为 RNA 合成的底物。由 1 个核糖和 3 个磷酸组成，核碱基和三磷酸基团与 1′和 5′碳。也可能参与细胞内的信号转导、能量传递、作为代谢反应中底物的激活剂或参与蛋白质合成和糖异生。
Other Notes
1 mM GTP = 13.7 A 252 单位
仅用于生命科学研究。不可用于诊断。
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Mfd Dynamically Regulates Transcription via a Release and Catch-Up Mechanism
Le TT, et al.
Cell (2017)
Cell-free Generation of COPII-coated Procollagen I Carriers
Yuan L, et al.
Bio-protocol, 7(22) (2017)
Youngseo Cheon et al.
Genome biology, 22(1), 294-294 (2021-10-20)
Promoter-proximal pausing of RNA polymerase II (RNAPII) is a critical step for the precise regulation of gene expression. Despite the apparent close relationship between promoter-proximal pausing and nucleosome, the role of chromatin remodeler governing this step has mainly remained elusive.
Riku Nagai et al.
Methods and protocols, 4(3) (2021-07-22)
The rates of translation elongation or termination in eukaryotes are modulated through cooperative molecular interactions involving mRNA, the ribosome, aminoacyl- and nascent polypeptidyl-tRNAs, and translation factors. To investigate the molecular mechanisms underlying these processes, we developed an in vitro translation
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