产品名称
DIG核酸检测试剂盒, sufficient for 40 blots (10 cm x 10 cm each), kit of 1 (5 components), suitable for hybridization, suitable for Northern blotting
usage
sufficient for 40 blots (10 cm x 10 cm each)
packaging
kit of 1 (5 components)
manufacturer/tradename
Roche
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable
greener alternative category
, Aligned
storage temp.
−20°C
Quality Level
Application
- Southern印迹
- Northern印迹
- 其他核酸印迹应用
- 原位杂交应用
- Southern印迹
- Northern印迹
- 其他核酸印迹应用
- 原位杂交应用
Biochem/physiol Actions
Features and Benefits
General description
Other Notes
Packaging
Preparation Note
偶联物的工作浓度取决于应用类型和底物。
储存条件(工作溶液):
- 抗-地高辛-AP缀合(瓶3):在2至8°C下可稳定保存12个月
- NBT/BCIP(瓶4):2至8°C下稳定
注意:在干冰上运输试剂盒过程中,可能发生沉淀,可通过短暂升温至37℃使其溶解 - 封闭试剂(瓶5)可在4至8°C下干燥储存,可稳定36个月。
- 经高压灭菌的储备溶液可以在15至25°C(未开封)或4至8°C(开封后)储存数天至一周。也可分液储存,在-15至-25°C条件下储存长达6个月。
仅试剂盒组分
- DIG-labeled Control DNA 5 µg/ml
- DNA Dilution Buffer, [50 μg/ml] fish sperm DNA
- Anti-Digoxigenin-AP Conjugate antibody 750 U/ml
- NBT/BCIP, 50x stock solution 50x concentrated
- Blocking Reagent
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
does not flashNot applicable
flash_point_c
does not flashNot applicable
法规信息
商品
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
地高辛(DIG)标记方法和试剂盒适用于DNA和RNA DIG探针、随机引物DNA标记、切口平移标记、5'和3'寡核苷酸末端标记。
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持