Merck
CN

11207741910

Roche

抗-地高辛-荧光素,Fab片段

from sheep

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别名:
地高辛配基, 抗-地高辛配基抗体

生物来源

sheep

质量水平

偶联物

fluorescein conjugate

抗体形式

purified immunoglobulin

antibody product type

primary antibodies

克隆

polyclonal

形式

lyophilized

包装

pkg of 200 μg

manufacturer/tradename

Roche

同位素/亚型

IgG

储存温度

2-8°C

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此商品
112146670011120773391011207750910
Anti-Digoxigenin, Fab fragments from sheep

Roche

11214667001

抗 地高辛,Fab 片段

Anti-Digoxigenin-POD, Fab fragments from sheep

Roche

11207733910

抗-地高辛-POD,Fab片段

conjugate

fluorescein conjugate

conjugate

unconjugated

conjugate

peroxidase conjugate

conjugate

rhodamine conjugate

antibody form

purified immunoglobulin

antibody form

affinity purified immunoglobulin

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody form

purified antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

form

lyophilized

form

lyophilized

form

lyophilized (clear, brown solution after reconstitution)

form

lyophilized (clear, red solution after reconstitution)

packaging

pkg of 200 μg

packaging

pkg of 1 mg

packaging

pkg of 150 U

packaging

pkg of 200 μg

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一般描述

抗地高辛-荧光素,Fab片段带有来自多克隆抗地高辛抗体的fab片段,并且与荧光素缀合。

特异性

来自绵羊的多克隆抗体对地高辛配基和地高辛具有特异性,并且与其他类固醇(如人类雌激素和雄激素)没有交叉反应性。

应用

抗地高辛-荧光素,Fab片段可用于检测地高辛标记的化合物:
  • 糖结合物研究中地高辛标记的糖
  • 荧光 原位 杂交(FISH)
  • 免疫组织细胞化学
  • 原位 杂交
抗地高辛-荧光素,Fab片段不可用于膜应用。

制备说明

  • 工作浓度:结合物的工作浓度取决于应用类型和底物。以下浓度可作为指导:检测糖蛋白中地高辛标记的糖:50至20μg/ml
  • 荧光原位 杂交(FISH):1至20μg/ml
  • 免疫组织细胞化学:20至50μg/ml
  • 原位 杂交:20至50μg/ml

抗DIG抗体具有高度特异性和敏感性。因此,对于FISH,1μg/ml的浓度已足够,并且仅在关键探针的情况下,该浓度可以升高至<10μg/ml(可能产生明显的背景信号)。
工作溶液:溶解
加入1ml双蒸水至终浓度为200μg/ml。

封闭:
PBS,0.5%牛血清白蛋白(w/v),pH7.4。
1%封闭试剂(w/v),1至5%热灭活的胎牛血清(v/v)或绵羊正常血清(可用于减少非特异性结合)。此外,pH可以增加至pH8.5至9.0。
储存条件(工作溶液):每次请制备新鲜溶液!
用地高辛免疫后,通过离子交换层析纯化绵羊IgG,并通过免疫吸附分离特异性IgG。通过木瓜蛋白酶消化获得的Fab片段,可通过凝胶过滤进行纯化,并与特异性标记缀合,并稳定于缓冲液中。

重悬

加入1ml双蒸水至终浓度为200μg/ml。

其他说明

仅用于生命科学研究。不可用于诊断。

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Aquatic Chronic 3 - Skin Sens. 1

储存分类代码

13 - Non Combustible Solids

WGK

WGK 2

闪点(°F)

does not flash

闪点(°C)

does not flash

法规信息

含少量动物源组分生物产品
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地高辛 (DIG) 标记方法

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