产品名称
抗-地高辛-荧光素，Fab片段, from sheep
biological source
sheep
conjugate
fluorescein conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized
packaging
pkg of 200 μg
manufacturer/tradename
Roche
isotype
IgG
storage temp.
2-8°C
Quality Level
Application
抗地高辛-荧光素，Fab片段可用于检测地高辛标记的化合物：
- 糖结合物研究中地高辛标记的糖
- 荧光 原位 杂交（FISH）
- 免疫组织细胞化学
- 原位 杂交
Biochem/physiol Actions
来自绵羊的多克隆抗体对地高辛配基和地高辛具有特异性，并且与其他类固醇（如人类雌激素和雄激素）没有交叉反应性。
General description
抗地高辛-荧光素，Fab片段带有来自多克隆抗地高辛抗体的fab片段，并且与荧光素缀合。
Other Notes
仅用于生命科学研究。不可用于诊断。
Preparation Note
- 工作浓度：结合物的工作浓度取决于应用类型和底物。以下浓度可作为指导：检测糖蛋白中地高辛标记的糖：50至20μg/ml
- 荧光原位 杂交(FISH)：1至20μg/ml
- 免疫组织细胞化学：20至50μg/ml
- 原位 杂交：20至50μg/ml
抗DIG抗体具有高度特异性和敏感性。因此，对于FISH，1μg/ml的浓度已足够，并且仅在关键探针的情况下，该浓度可以升高至<10μg/ml（可能产生明显的背景信号）。
工作溶液：溶解
加入1ml双蒸水至终浓度为200μg/ml。
封闭：
PBS，0.5％牛血清白蛋白(w/v)，pH7.4。
1％封闭试剂(w/v)，1至5％热灭活的胎牛血清(v/v)或绵羊正常血清（可用于减少非特异性结合）。此外，pH可以增加至pH8.5至9.0。
储存条件（工作溶液）：每次请制备新鲜溶液！
加入1ml双蒸水至终浓度为200μg/ml。
用地高辛免疫后，通过离子交换层析纯化绵羊IgG，并通过免疫吸附分离特异性IgG。通过木瓜蛋白酶消化获得的Fab片段，可通过凝胶过滤进行纯化，并与特异性标记缀合，并稳定于缓冲液中。
未找到合适的产品？
试试我们的产品选型工具.
signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 3 - Skin Sens. 1
存储类别
13 - Non Combustible Solids
wgk
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
常规特殊物品
此项目有
Evidence of Interspecific Chromosomal Diversification in Rainbowfishes (Melanotaeniidae, Teleostei).
Zuzana Majtánová et al.
Genes, 11(7) (2020-07-28)
Rainbowfishes (Melanotaeniidae) are the largest monophyletic group of freshwater fishes occurring in Australia and New Guinea, with 112 species currently recognised. Despite their high taxonomic diversity, rainbowfishes remain poorly studied from a cytogenetic perspective. Using conventional (Giemsa staining, C banding
Caroline Scott et al.
Haematologica, 106(11), 2960-2970 (2020-10-31)
The investigation of inherited disorders of erythropoiesis has elucidated many of the principles underlying the production of normal red blood cells and how this is perturbed in human disease. Congenital Dyserythropoietic Anaemia type 1 (CDA-I) is a rare form of
Hasan Yardimci et al.
Methods (San Diego, Calif.), 57(2), 179-186 (2012-04-17)
The recent advent in single-molecule imaging and manipulation methods has made a significant impact on the understanding of molecular mechanisms underlying many essential cellular processes. Single-molecule techniques such as electron microscopy and DNA fiber assays have been employed to study
Ritu Bhalla et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 26(6), 835-848 (2013-01-26)
Identification of new molecular markers has led to the molecular classification of prostate cancer based on driving genetic lesions. The translation of these discoveries for clinical use necessitates the development of simple, reliable and rapid detection systems to screen patients
Xiufen Zheng et al.
Oncogenesis, 9(3), 34-34 (2020-03-14)
The underlying mechanisms of long non-coding RNAs (lncRNA) participating in the progression of lung cancers are largely unknown. We found a novel lncRNA, PIK3CD antisense RNA 2 (PIK3CD-AS2), that contributes to lung adenocarcinoma (LUAD) progression. The expression characteristics of PIK3CD-AS2
商品
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
地高辛（DIG）标记方法和试剂盒适用于DNA和RNA DIG探针、随机引物DNA标记、切口平移标记、5'和3'寡核苷酸末端标记。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持