产品名称
抗-地高辛, from sheep
biological source
sheep
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized
packaging
pkg of 200 μg
manufacturer/tradename
Roche
isotype
IgG
storage temp.
2-8°C
Quality Level
Analysis Note
与其他类固醇无交叉反应，如人体雌激素（例如雌二醇）或雄激素（例如睾丸激素）。
Application
使用抗地高辛抗体检测地高辛标记的化合物，所用方法：
- ELISA
- 免疫组织细胞化学
- 原位杂交
- 蛋白质印迹
抗地高辛配基已用于DNA束缚。其已被用于将DNA分子附着到流动池的玻璃表面上。
Biochem/physiol Actions
存在Na+、Mg2+和ATP的情况下，地高辛可抑制钠离子泵。
来自绵羊的多克隆抗体对地高辛配基和地高辛具有特异性，并且与其他类固醇（如人类雌激素和雄激素）没有交叉反应性。
General description
与放射性探针相比，地高辛标记探针具有更高的灵敏度。可以更快地进行检测，危险性更低，保质期更长。
地高辛是一种半抗原，可用于核酸标记和检测系统。
Other Notes
仅用于生命科学研究。不可用于诊断。
Preparation Note
工作浓度：抗体的工作浓度取决于应用类型和底物。以下浓度可作为指导：
工作溶液：用于包膜应用：PBS（磷酸盐缓冲盐水），pH 7.4
- ELISA：适于包膜：2至4μg/ml
- 免疫组织细胞化学：0.5至2μg/ml
- 原位 杂交：0.2至0.4μg/ ml
- 蛋白质印迹：0.5至2μg/ml
工作溶液：用于包膜应用：PBS（磷酸盐缓冲盐水），pH 7.4
加入1ml PBS至终浓度为200μg/ml。
用地高辛免疫后，通过离子交换层析纯化绵羊IgG，并通过免疫吸附分离特异性IgG。
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signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
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商品
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
地高辛（DIG）标记方法和试剂盒适用于DNA和RNA DIG探针、随机引物DNA标记、切口平移标记、5'和3'寡核苷酸末端标记。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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