一般描述
SuRE/Cut缓冲液体系是由5种优化孵育缓冲液组成的10倍浓缩液,适用于DNA限制性酶切消化。已经测定了每种缓冲液中所有限制酶的活性,以选择100%活性或计算双倍消化的活性。
应用
SuRE/Cut™缓冲液H已用于处理EcoRI和XhoI限制性内切酶,用于消化凝胶纯化的反应产物和pMX-IG载体。还用于稀释巴氏黄单胞菌(Xanthomonas badrii)限制性内切酶I(Xba I),用于消化大肠杆菌菌落基因组DNA和布伦登卢普沙门菌(Salmonella Braenderup)H2812 (ATCC BAA-664)分子量标准。
SuRE/Cut缓冲液H已用于基因组DNA的限制性内切酶XbaI消化。还用于PCR 产物和质粒的EcoRI和XhoI酶切消化。
其他说明
仅用于生命科学研究。不可用于诊断。
法律信息
SuRE/Cut is a trademark of Roche
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
No data available
闪点(°C)
No data available
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商品
The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.
术语“限制酶”源自Werner Arber和Matthew Meselson实验室对肠杆菌噬菌体λ(λ噬菌体)的研究。
相关内容
Restriction endonucleases in prokaryotes function primarily to protect against foreign genetic material, notably bacteriophage DNA.
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