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Merck
CN

11467140001

Roche

DNA,MB级

from fish sperm

别名:

DNA

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关于此项目

UNSPSC Code:
41106310
NACRES:
NA.54
Biological source:
fish (sperm)
Form:
solution (ready-to-use)
Solubility:
water: miscible
Storage temp.:
−20°C
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biological source

fish (sperm)

grade

Molecular Biology

form

solution (ready-to-use)

mol wt

40-1,000 kDa

packaging

pkg of 500 mg

manufacturer/tradename

Roche

concentration

100 μg/mL

color

light yellowopalescent

solubility

water: miscible

storage temp.

−20°C

General description

MB级DNA是以超声的变性单链DNA片段混合物形式提供的。
MB级DNA被用于防止非特异性杂交反应。

Application

该即用型溶液是直接添加到杂交混合液中的。它可防止在所有类型的膜(Southern和Northern blot应用)或原位DNA杂交中发生非特异性杂交。MB级的DNA已被用于染色质免疫沉淀(ChiP)分析。
注:加入NaCl、超声或打断以及变性都不是必需的。

Physical form

溶液,10 mg/ml ,10 mM Tris-HCl,10 mM NaCl,1 mM EDTA,pH 8.0中

Preparation Note

工作浓度:100μg/ml
该鱼精DNA应在预杂交溶液中以100 μg/ml(1:100稀释)的浓度进行使用。

Other Notes

仅用于生命科学研究。不可用于诊断。
链长为 120 至 3,000 个核苷酸


存储类别

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

No data available

flash_point_c

No data available

法规信息

低风险生物材料

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Yu Sasano et al.
Bio-protocol, 7(10), e2306-e2306 (2017-05-20)
Chromosome engineering is an important technology with applications in basic biology and biotechnology. Chromosome splitting technology called PCS (PCR-mediated Chromosome Splitting) has already been developed as a fundamental chromosome engineering technology in the budding yeast. However, the splitting efficiency of
Ryudo Ohbayashi et al.
Bio-protocol, 8(15), e2958-e2958 (2018-08-05)
Cyanobacteria are prokaryotic organisms that carry out oxygenic photosynthesis. The fresh water cyanobacterium Synechococcus elongatus PCC 7942 is a model organism for the study of photosynthesis and gene regulation, and for biotechnological applications. Besides several freshwater cyanobacteria, S. elongatus 7942
Jasmin van den Heuvel et al.
eLife, 10 (2021-07-29)
In humans and other holozoan organisms, the ribosomal protein eS30 is synthesized as a fusion protein with the ubiquitin-like protein FUBI. However, FUBI is not part of the mature 40S ribosomal subunit and cleaved off by an as-of-yet unidentified protease.



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货号GTIN
1146714000104061838702852